Micropropagation of Achyranthes japonica through axillary buds culture

Multiple shoot formation was obtained from excised axillary buds of Achyranthes japonica N-AKAI cultured on Murashige & Skoog (MS) media containing various growth regulators such as auxin and cytokinin. The highest average number of shoots was obtained in 1 mg/l alpha-naphthalene acetic acid (NAA) and 2 mg/l 6-benzyladenine (BA) after 6 weeks (25.8 adventitious shoots per node). Although the regeneration rate was less than the former condition, optimal combination for the production of more shoots with a suitable size was 0.5 mg/l NAA and 1.0 mg/l BA (19.7 adventitious shoots per node). Roots were induced from regenerated shoots after 3 weeks culture, transferred to 1/2 MS medium supplemented with 0.1 mg/l indole butyric acid (IBA). Micropropagated plants were successfully transferred to soil.