Background: The chondrogenic differentiation potential of the easily accessible adipose tissue-derived stem cells (ASCs) is of particular interest within the field of tissue engineering for treating cartilage defects. However, no consensus has been reached as to which oxygen tension is more beneficial for the differentiation process. Materials & methods: In this investigation, the impact of available oxygen was investigated to identify optimal conditions for human ASC chondrogenesis in vitro. Four physiologically relevant oxygen concentrations of 15, 10, 5 and 1% were compared with ambient air condition, and the ASCs originating from six unrelated donors were subjected to chondrogenic induction in high-density pellet cultures. Results: The qualitative and quantitative assessment of accumulated extracellular matrix and the gene-expression analysis revealed marked interindividual differences, nevertheless the chondrogenic process was optimally supported in high-density pellet setup at ambient or 15% oxygen concentrations, irrespective of the origin of cells. The histochemical analysis based on alcian blue staining demonstrated that the differentiation took place in a gradient-like fashion, displaying highest levels in restricted regions, most often adjacent to the periphery. The two lowest hypoxic conditions, at 5 and 1% oxygen, seemed to have an inhibitory effect. Conclusion: The micropellet cultures at ambient or 15% oxygen concentration provided the most suitable environment for inducing chondrogenesis in ASCs. Furthermore, in light of the fact that the induction appeared in a zone-dependent manner, this format lends itself as a suitable model for further analysis of the relationship between chondrogenic differentiation and the gradient of nutrients.
机构:
Univ Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
Lin, Ching-Shwun
Xin, Zhong-Cheng
论文数: 0引用数: 0
h-index: 0
机构:
Peking Univ, Androl Ctr, Hosp 1, Beijing 100871, Peoples R ChinaUniv Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
Xin, Zhong-Cheng
Deng, Chun-Hua
论文数: 0引用数: 0
h-index: 0
机构:
Sun Yat Sen Univ, Affiliated Hosp 1, Dept Urol, Guangzhou 510275, Guangdong, Peoples R ChinaUniv Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
Deng, Chun-Hua
Ning, Hongxiu
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
Ning, Hongxiu
Lin, Guiting
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
Lin, Guiting
Lue, Tom F.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
机构:
China Med Univ, Shengjing Hosp, Dept Blood Transfus, Shenyang 110004, Peoples R ChinaChina Med Univ, Shengjing Hosp, Dept Blood Transfus, Shenyang 110004, Peoples R China
Wang, Zhe
Li, Hongqiu
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Shengjing Hosp, Dept Orthopaed, Shenyang 110004, Peoples R China
Cent Hosp, Shenyang Med Coll, Dept Orthoped, Shenyang 110024, Peoples R ChinaChina Med Univ, Shengjing Hosp, Dept Blood Transfus, Shenyang 110004, Peoples R China
Li, Hongqiu
Zhang, Dianbao
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Dept Stem Cells & Regenerat Med, Key Lab Cell Biol, Minist Publ Hlth, Shenyang 110001, Peoples R China
China Med Univ, Key Lab Med Cell Biol, Minist Educ, Shenyang 110001, Peoples R ChinaChina Med Univ, Shengjing Hosp, Dept Blood Transfus, Shenyang 110004, Peoples R China
Zhang, Dianbao
Liu, Xiaoyu
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Dept Stem Cells & Regenerat Med, Key Lab Cell Biol, Minist Publ Hlth, Shenyang 110001, Peoples R China
China Med Univ, Key Lab Med Cell Biol, Minist Educ, Shenyang 110001, Peoples R ChinaChina Med Univ, Shengjing Hosp, Dept Blood Transfus, Shenyang 110004, Peoples R China
Liu, Xiaoyu
Zhao, Feng
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Dept Stem Cells & Regenerat Med, Key Lab Cell Biol, Minist Publ Hlth, Shenyang 110001, Peoples R China
China Med Univ, Key Lab Med Cell Biol, Minist Educ, Shenyang 110001, Peoples R ChinaChina Med Univ, Shengjing Hosp, Dept Blood Transfus, Shenyang 110004, Peoples R China
Zhao, Feng
Pang, Xining
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Dept Stem Cells & Regenerat Med, Key Lab Cell Biol, Minist Publ Hlth, Shenyang 110001, Peoples R China
China Med Univ, Key Lab Med Cell Biol, Minist Educ, Shenyang 110001, Peoples R ChinaChina Med Univ, Shengjing Hosp, Dept Blood Transfus, Shenyang 110004, Peoples R China
Pang, Xining
Wang, Qiushi
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Shengjing Hosp, Dept Blood Transfus, Shenyang 110004, Peoples R ChinaChina Med Univ, Shengjing Hosp, Dept Blood Transfus, Shenyang 110004, Peoples R China
机构:
Comenius Univ, Inst Med Biol Genet & Clin Genet, Fac Med, SK-81108 Bratislava, SlovakiaComenius Univ, Inst Med Biol Genet & Clin Genet, Fac Med, SK-81108 Bratislava, Slovakia
Danisovic, L'ubos
Kuniakova, Marcela
论文数: 0引用数: 0
h-index: 0
机构:
Comenius Univ, Inst Med Biol Genet & Clin Genet, Fac Med, SK-81108 Bratislava, SlovakiaComenius Univ, Inst Med Biol Genet & Clin Genet, Fac Med, SK-81108 Bratislava, Slovakia
Kuniakova, Marcela
Varchulova-Novakova, Zuzana
论文数: 0引用数: 0
h-index: 0
机构:
Comenius Univ, Inst Med Biol Genet & Clin Genet, Fac Med, SK-81108 Bratislava, SlovakiaComenius Univ, Inst Med Biol Genet & Clin Genet, Fac Med, SK-81108 Bratislava, Slovakia
Varchulova-Novakova, Zuzana
Bohac, Martin
论文数: 0引用数: 0
h-index: 0
机构:
Comenius Univ, Clin Plast Surg, Fac Med, SK-82101 Bratislava, Slovakia
Comenius Univ, Fac Med, Inst Histol & Embryol, Bratislava 81108, SlovakiaComenius Univ, Inst Med Biol Genet & Clin Genet, Fac Med, SK-81108 Bratislava, Slovakia
Bohac, Martin
Varga, Ivan
论文数: 0引用数: 0
h-index: 0
机构:
Comenius Univ, Fac Med, Inst Histol & Embryol, Bratislava 81108, Slovakia
Slovak Med Univ, Inst Histol & Embryol, Fac Med, SK-83303 Bratislava, SlovakiaComenius Univ, Inst Med Biol Genet & Clin Genet, Fac Med, SK-81108 Bratislava, Slovakia
Varga, Ivan
Rusnakova, Jana
论文数: 0引用数: 0
h-index: 0
机构:
Comenius Univ, Fac Med, Inst Histol & Embryol, Bratislava 81108, SlovakiaComenius Univ, Inst Med Biol Genet & Clin Genet, Fac Med, SK-81108 Bratislava, Slovakia
Rusnakova, Jana
Polak, Stefan
论文数: 0引用数: 0
h-index: 0
机构:
Comenius Univ, Fac Med, Inst Histol & Embryol, Bratislava 81108, SlovakiaComenius Univ, Inst Med Biol Genet & Clin Genet, Fac Med, SK-81108 Bratislava, Slovakia
Polak, Stefan
Boehmer, Daniel
论文数: 0引用数: 0
h-index: 0
机构:
Comenius Univ, Inst Med Biol Genet & Clin Genet, Fac Med, SK-81108 Bratislava, SlovakiaComenius Univ, Inst Med Biol Genet & Clin Genet, Fac Med, SK-81108 Bratislava, Slovakia
机构:
Butantan Inst, Lab Genet, Ave Vital Brasil 1500, BR-05503900 Sao Paulo, SP, Brazil
Univ Fed Sao Paulo, R Sena Madureira 1500, BR-04021001 Sao Paulo, SP, BrazilButantan Inst, Lab Genet, Ave Vital Brasil 1500, BR-05503900 Sao Paulo, SP, Brazil
Santos Rizzo Zuttion, Marilia Sanches
Dias Camara, Diana Aparecida
论文数: 0引用数: 0
h-index: 0
机构:
Butantan Inst, Lab Genet, Ave Vital Brasil 1500, BR-05503900 Sao Paulo, SP, Brazil
Univ Fed Sao Paulo, R Sena Madureira 1500, BR-04021001 Sao Paulo, SP, BrazilButantan Inst, Lab Genet, Ave Vital Brasil 1500, BR-05503900 Sao Paulo, SP, Brazil
Dias Camara, Diana Aparecida
Dariolli, Rafael
论文数: 0引用数: 0
h-index: 0
机构:
Univ Sao Paulo, Med Sch, Heart Inst InCor, Brazil Ave Dr Eneas de Carvalho Aguiar,44, BR-05403900 Sao Paulo, SP, BrazilButantan Inst, Lab Genet, Ave Vital Brasil 1500, BR-05503900 Sao Paulo, SP, Brazil
Dariolli, Rafael
Takimura, Celso
论文数: 0引用数: 0
h-index: 0
机构:
Univ Sao Paulo, Med Sch, Heart Inst InCor, Brazil Ave Dr Eneas de Carvalho Aguiar,44, BR-05403900 Sao Paulo, SP, BrazilButantan Inst, Lab Genet, Ave Vital Brasil 1500, BR-05503900 Sao Paulo, SP, Brazil
Takimura, Celso
Wenceslau, Cristiane
论文数: 0引用数: 0
h-index: 0
机构:
Butantan Inst, Lab Genet, Ave Vital Brasil 1500, BR-05503900 Sao Paulo, SP, BrazilButantan Inst, Lab Genet, Ave Vital Brasil 1500, BR-05503900 Sao Paulo, SP, Brazil
Wenceslau, Cristiane
Kerkis, Irina
论文数: 0引用数: 0
h-index: 0
机构:
Butantan Inst, Lab Genet, Ave Vital Brasil 1500, BR-05503900 Sao Paulo, SP, Brazil
Univ Fed Sao Paulo, R Sena Madureira 1500, BR-04021001 Sao Paulo, SP, BrazilButantan Inst, Lab Genet, Ave Vital Brasil 1500, BR-05503900 Sao Paulo, SP, Brazil