Sex determination by PCR analysis of DNA extracted from incinerated, deciduous teeth

被引：8
|
作者：
Williams, D
Lewis, M
Franzen, T
Lissett, V
Adams, C
Whittaker, D
Tysoe, C
Butler, R
机构：
[1] Cardiff Univ, Coll Med, Dept Oral Surg Med & Pathol, Cardiff CF14 4XY, S Glam, Wales
[2] Cardiff Univ, Coll Med, Forens Dent Unit, Univ Dent Sch, Cardiff CF14 4XY, S Glam, Wales
[3] Cardiff Univ, Coll Med, Inst Med Genet, Cardiff CF14 4XY, S Glam, Wales
来源：
SCIENCE & JUSTICE | 2004年 / 44卷 / 02期
关键词：
forensic science; DNA; sex identification; deciduous teeth; amelogenin gene; PCR;
D O I：
10.1016/S1355-0306(04)71694-9
中图分类号：
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号：
0301 ; 10 ;
摘要：
Establishing the biological sex of human remains is a very important part of identifying victims of fire when severe soft tissue destruction has occurred. Deciduous (children's) teeth were exposed to a range of incineration temperatures 100-500degreesC for 15 minutes. Polymerase Chain Reaction (PCR) amplification was used to identify specific human amelogenin regions. There was successful identification of human biological sex, from deciduous teeth exposed to incineration temperatures of 200degreesC and below, using standard ethidium bromide get staining. There was greater sensitivity using fragment analysis by laser induced fluorescence which achieved sex identification from some teeth heated to 400degreesC.
引用
收藏
页码：89 / 94
页数：6
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