Hyperthermia decreases cytokine-mediated adhesion molecule expression on human umbilical vein endothelial cells

被引:10
作者
Brand, K
Lubbe, AS
Justus, DJ
机构
[1] UNIV LOUISVILLE,SCH MED,CTR APPL MICROCIRCULATORY RES,LOUISVILLE,KY 40292
[2] FREE UNIV BERLIN,DEPT HEMATOL ONCOL,D-1000 BERLIN,GERMANY
关键词
hyperthermia; adhesion molecules; cytokines; metastasis and endothelial cells;
D O I
10.3109/02656739609023529
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Although hyperthermia has been used as an effective cancer treatment modality, its effects on metastasis of tumour cells are not clear. Since adhesion molecules play a key role in metastasis: we evaluated how the expression of adhesion molecules is influenced by hyperthermia. Human umbilical vein endothelial cells were incubated in vitro for 1 h. at 39, 42, 43 and 44 degrees C with and without addition of tumour-necrosis factor (TNF) or interferon-gamma (IFN-gamma) and the expression of endothelial cell leukocyte adhesion molecule-1 (ELAM-1), intercellular adhesion molecule-1 (ICAM-1) and major histocompatibility complex (MHC) class-II molecule was measured. Expression of MHC class-II molecules and expression of unstimulated constituent ICAM-1's was not reduced by heat treatment. In contrast, expression of cytokine-induced ELAM-1's and ICAM-1's was significantly lower after heat treatment. The adhesion to HUVEC in vitro of HL-60 leukemia cells, which express sialyl-Lewis-x antigen as a ligand to ELAM-1, was diminished after incubation at 42 degrees C and totally lost after treatment at 44 degrees C. This suggests that any decrease in metastasis formation after heat treatment, which is occasionally observed, could be due to a reduced action of TNF or related cytokines on adhesion molecule induction and subsequent membrane expression by the endothelial cell. A possible underlying mechanism involved is a heat-induced alteration or blockage of the biosynthetic pathways required for synthesis of ELAM-1 and ICAM-1 proteins.
引用
收藏
页码:527 / 538
页数:12
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