MiR-455-3p regulates early chondrogenic differentiation via inhibiting Runx2

被引：47

作者：

Zhang, Zhiqi ^{[1
]}

Hou, Changhe ^{[1
]}

Meng, Fangang ^{[1
]}

Zhao, Xiaoyi ^{[1
]}

Zhang, Ziji ^{[1
]}

Huang, Guangxin ^{[1
]}

Chen, Weishen ^{[1
]}

Fu, Ming ^{[1
]}

Liao, Weiming ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Joint Surg, Guangzhou 510080, Guangdong, Peoples R China

来源：

FEBS LETTERS | 2015年 / 589卷 / 23期

关键词：

Chondrogenesis; MiR-455-3p; Runt-related transcription factor 2; TRANSCRIPTION FACTOR RUNX2; MESENCHYMAL STEM-CELLS; ENDOCHONDRAL OSSIFICATION; CHONDROCYTE PROLIFERATION; SKELETAL DEVELOPMENT; EXPRESSION; MICRORNAS; OSTEOARTHRITIS; HYPERTROPHY; MATURATION;

D O I：

10.1016/j.febslet.2015.09.032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The expression of miR-455-3p has been shown to be up-regulated in chondrogenesis of mesenchymal stem cell, but its role in different stages during chondrogenesis remains unknown. Here, we show that miR-455-3p is increased in ATDC5 cells from 0 d to 21 d, but rapidly decreases at 28 d, and a similar expression kinetic is detected in the development of mouse embryos. We show that miR-455-3p functions as an activator for early chondrogenic differentiation, most likely by inhibiting the expression of Runt-related transcription factor 2 (Runx2) as indicated by luciferase reporter assays. In conclusion, miR-455-3p may activate early chondrogenesis by directly targeting Runx2. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3671 / 3678

页数：8

共 40 条

[1] bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila [J].