Osmotic stress leads to decreased intracellular pH of Listeria monocytogenes as determined by fluorescence ratio-imaging microscopy

被引:12
作者
Fang, WH
Siegumfeldt, H
Budde, BB
Jakobsen, M
机构
[1] Royal Vet & Agr Univ, Dept Dairy & Food Sci, DK-1958 Frederiksberg C, Denmark
[2] Zhejiang Univ, Coll Anim Sci, Hangzhou 310029, Zhejiang, Peoples R China
关键词
D O I
10.1128/AEM.70.5.3176-3179.2004
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Intracellular pH (pH(i)) of Listeria monocytogenes was determined after exposure to NaCl or sorbitol in liquid and solid media (agar). Both compounds decreased pHi, and recovery on solid medium was impaired compared to that in liquid medium. N,N'-dicyclohexylcarbodiimide abolished pH(i) recovery, and lowering a(w) with glycerol showed no effect on pH(i).
引用
收藏
页码:3176 / 3179
页数:4
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