Cyclooxygenase-2 Expression in Pigs Infected Experimentally with Mycoplasma hyopneumoniae

被引：6
作者：
Andrada, M. [1 ]
Quesada-Canales, O. [1 ]
Suarez-Bonnet, A. [1 ]
Paz-Sanchez, Y. [1 ]
Espinosa de los Monteros, A. [1 ]
Rodriguez, F. [1 ]
机构：
[1] Univ Las Palmas Gran Canaria, Sch Vet, Inst Anim Hlth, Unit Vet Histol & Pathol, Arucas 35413, Gran Canaria, Spain
来源：
JOURNAL OF COMPARATIVE PATHOLOGY | 2014年 / 151卷 / 2-3期
关键词：
cyclooxygenase-2; Mycoplasma hyopneumoniae; pig; pneumonia; ENZOOTIC PNEUMONIA; NEUTROPHILS; LUNGS;
D O I：
10.1016/j.jcpa.2014.04.005
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
Porcine enzootic pneumonia, primarily caused by Mycoplasma hyopneumoniae (Mh), is a contagious disease characterized by catarrhal bronchointerstitial pneumonia. Previous studies have evaluated immunohistochemically the distribution of Mb, different cellular populations and cytokines during Mb-induced pneumonia. Cyclooxygenase (COX)-2 is overexpressed during inflammatory responses by different cell types in the lung. The aim of this study was to elucidate the possible role of COX-2 in the pathogenesis of porcine enzootic pneumonia. COX-2 protein was detected by immunohistochemistry in formalin-fixed, paraffin wax-embedded lung tissues from 10 pigs infected experimentally with Mh. Ten pigs were inoculated intranasally with Mh and killed in pairs weekly from 1 to 5 weeks post inoculation. Three Mh-free pigs were taken as controls. Bronchial and bronchiolar epithelial cells, bronchial submucosal glands and a small number of macrophages in the bronchoalveolar exudate expressed COX-2. COX-2 protein was always associated with areas of pneumonia and expression was minimal in lungs from control pigs. These results suggest that COX-2 plays a role in the pathogenesis of Mh-infection. (C) 2014 Elsevier Ltd. All rights reserved.
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收藏
页码：271 / 276
页数：6
相关论文
共 16 条
[1]   Suppressive effect of bronchoalveolar lavage fluid from pigs infected with Mycoplasma hyopneumoniae on chemiluminescence of porcine peripheral neutrophils [J].
Asai, T ;
Okada, M ;
Yokomizo, Y ;
Sato, S ;
Mori, Y .
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1996, 51 (3-4) :325-331
[2]   DETECTION OF INTERLEUKIN-6 AND PROSTAGLANDIN-E(2) IN BRONCHOALVEOLAR LAVAGE FLUIDS OF PIGS EXPERIMENTALLY INFECTED WITH MYCOPLASMA-HYOPNEUMONIAE [J].
ASAI, T ;
OKADA, M ;
ONO, M ;
MORI, Y ;
YOKOMIZO, Y ;
SATO, S .
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1994, 44 (01) :97-102
[3]   Expression of nitric oxide synthase 2 and cyclooxygenase-2 in swine experimentally infected with Actinobacillus pleuropneumoniae [J].
Cho, WS ;
Chae, C .
VETERINARY PATHOLOGY, 2004, 41 (06) :666-672
[4]   5-Lipoxygenase and cyclooxygenase-2 in the lungs of pigs naturally affected by enzootic pneumonia and porcine pleuropneumonia [J].
Giacominelli-Stuffler, R. ;
Marruchella, G. ;
Storelli, M. M. ;
Sabatucci, A. ;
Angelucci, C. B. ;
Maccarrone, M. .
RESEARCH IN VETERINARY SCIENCE, 2012, 93 (02) :898-903
[5]   Interleukin-1β-induced mucin production in human airway epithelium is mediated by cyclooxygenase-2, prostaglandin E2 receptors, and cyclic AMP-protein kinase A signaling [J].
Gray, T ;
Nettesheim, P ;
Loftin, C ;
Koo, JS ;
Bonner, J ;
Peddada, S ;
Langenbach, R .
MOLECULAR PHARMACOLOGY, 2004, 66 (02) :337-346
[6]  
HEMPEL SL, 1994, J BIOL CHEM, V269, P32979
[7]   Expression of secreted and membrane-bound mucins in the airways of piglets experimentally infected with Mycoplasma hyopneumoniae [J].
Kim, C. H. ;
Oh, Y. ;
Han, K. ;
Seo, H. W. ;
Kim, D. ;
Park, C. ;
Kang, I. ;
Chae, C. .
VETERINARY JOURNAL, 2012, 192 (01) :120-122
[8]  
Kumar V., 2007, ROBBINS BASIC PATHOL, V8th, P31
[9]   Chronologic localization of Mycoplasma hyopneumoniae in experimentally infected pigs [J].
Kwon, D ;
Choi, C ;
Chae, C .
VETERINARY PATHOLOGY, 2002, 39 (05) :584-587
[10]  
Livingston DW, 1972, AM J VET RES, V33, P2249
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