Urinary bacteria in adult women with urgency urinary incontinence

被引：83

作者：

Brubaker, Linda ^{[1
,2
]}

Nager, Charles W. ^{[3
]}

Richter, Holly E. ^{[4
]}

Visco, Anthony ^{[5
]}

Nygaard, Ingrid ^{[6
]}

Barber, Matthew D. ^{[7
]}

Schaffer, Joseph ^{[8
]}

Meikle, Susan ^{[9
]}

Wallace, Dennis ^{[10
]}

Shibata, Noriko ^{[11
]}

Wolfe, Alan J. ^{[11
]}

机构：

[1] Loyola Univ Chicago, Stritch Sch Med, Dept Obstet & Gynecol, Maywood, IL 60153 USA

[2] Loyola Univ Chicago, Stritch Sch Med, Dept Urol, Maywood, IL 60153 USA

[3] UC San Diego Hlth Syst, Dept Reprod Med, San Diego, CA USA

[4] Univ Alabama Birmingham, Dept Obstet & Gynecol, Birmingham, AL 35294 USA

[5] Duke Univ, Med Ctr, Dept Obstet & Gynecol, Durham, NC 27710 USA

[6] Univ Utah, Dept Obstet & Gynecol, Salt Lake City, UT USA

[7] Cleveland Clin, Obstet Gynecol & Womens Hlth Inst, Cleveland, OH 44106 USA

[8] Univ Texas SW Med Ctr Dallas, Dept Obstet & Gynecol, Dallas, TX 75390 USA

[9] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Gynecol Hlth & Dis Branch, NIH, Bethesda, MD USA

[10] Res Triangle Inst, Div Hlth Sci, Res Triangle Pk, NC 27709 USA

[11] Loyola Univ Chicago, Stritch Sch Med, Dept Microbiol & Immunol, Maywood, IL 60153 USA

来源：

INTERNATIONAL UROGYNECOLOGY JOURNAL | 2014年 / 25卷 / 09期

关键词：

Microbiome; Urinary bacteria; Urinary urgency incontinence; Urinary tract infection; 16S RDNA;

D O I：

10.1007/s00192-013-2325-2

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

This study's aims were to detect and quantify bacterial DNA in the urine of randomized trial participants about to undergo treatment for urinary urgency incontinence (UUI) without clinical evidence of urinary tract infection (UTI) and to determine if the presence of bacterial DNA in baseline urine relates to either baseline urinary symptoms or UTI risk after urinary tract instrumentation. Women without clinical evidence of baseline UTI were randomized to cystoscopic onabotulinum toxin A injection and oral placebo medication versus cystoscopic placebo injection and active oral medication. Bacterial DNA in participants' catheterized urine was measured by quantitative polymerase chain reaction (qPCR). Bacterial DNA was detected in the urine of 38.7 % of participants (60 out of 155). In these 60 qPCR-positive participants, baseline daily UUI episodes were greater than in the 95 qPCR-negative participants (5.71 [+/- 2.60] vs 4.72 [+/- 2.86], p = 0.004). Neither symptom severity by questionnaire nor treatment outcome was associated with qPCR status or with qPCR level in qPCR-positive subjects. In contrast, the presence of urinary bacterial DNA was associated with UTI risk: only 10 % of the qPCR-positive women developed a UTI post-treatment, while 24 % of the qPCR-negative women did so. The median qPCR level for qPCR-positive samples did not differ significantly by UTI status (UTI 2.58 x 10(5) vs no UTI 1.35 x 10(5) copies/mL, p = 0.6). These results may indicate a urinary bacterial contribution to both baseline UUI and the risk of post-treatment UTI.

引用

页码：1179 / 1184

页数：6

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