Performance of a commercial rapid dengue NS1 antigen immunochromatography test with reference to dengue NS1 antigen-capture ELISA

被引：55
作者：
Zainah, S. [1 ]
Wahab, A. H. Abdul [2 ]
Mariam, M. [2 ]
Fauziah, M. K. [1 ]
Khairul, A. H. [2 ]
Roslina, I. [1 ]
Sairulakhma, A. [1 ]
Kadimon, S. S. [2 ]
Jais, M. S. Mat [2 ]
Chua, K. B. [2 ]
机构：
[1] Inst Med Res, Infect Dis Res Ctr, Virol Unit, Kuala Lumpur 50588, Malaysia
[2] Minist Hlth, Natl Publ Hlth Lab, Sungai Buloh 47000, Selangor, Malaysia
来源：
JOURNAL OF VIROLOGICAL METHODS | 2009年 / 155卷 / 02期
关键词：
Dengue; Rapid Antigen Detection; Diagnosis; Immunochromatography; NONSTRUCTURAL GLYCOPROTEIN NS1; BORNE ENCEPHALITIS-VIRUS; JAPANESE ENCEPHALITIS; HEMORRHAGIC-FEVER; PROTEIN NS1; CELLS; DIAGNOSIS; SECRETION; ANTIBODY; SERA;
D O I：
10.1016/j.jviromet.2008.10.016
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The performance of a commercial immunochromatography test for rapid detection of dengue NS1 antigen present in serum or plasma of patients was evaluated against a commercial dengue NS1 antigen-capture ELISA. The rapid immunochromatography test gave an overall sensitivity of 90.4% with a specificity of 99.5%. The sensitivity was highest for serum samples from which virus was isolated (96.3%) and lowest for those from which virus was not isolated and RT-PCR was negative (76.4%). The sensitivity was significantly higher for serum samples from patients with acute primary dengue (92.3%) than those from patients with acute secondary dengue (79.1%). The positive predictive value and negative predictive value of this commercial immunochromatography test were 99.6% and 87.9% respectively. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：157 / 160
页数：4
相关论文
共 50 条
  • [1] Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection
    Kumarasamy, V.
    Wahab, A. H. Abdul
    Chua, S. K.
    Hassan, Z.
    Chem, Y. K.
    Mohamad, M.
    Chua, K. B.
    JOURNAL OF VIROLOGICAL METHODS, 2007, 140 (1-2) : 75 - 79
  • [2] Clinical Evaluation of the NS1 Antigen-Capture ELISA for Early Diagnosis of Dengue Virus Infection in Brazil
    Castro-Jorge, Luiza A.
    Machado, Paula R. L.
    Favero, Camila A.
    Borges, Marcos C.
    Passos, Luzia M. R.
    de Oliveira, Rosana Moreira
    Fonseca, Benedito A. L.
    JOURNAL OF MEDICAL VIROLOGY, 2010, 82 (08) : 1400 - 1405
  • [3] Dengue NS1 antigen as a marker of severe clinical disease
    Paranavitane, Shiran Ajith
    Gomes, Laksiri
    Kamaladasa, Achala
    Adikari, Thiruni N.
    Wickramasinghe, Nilanka
    Jeewandara, Chandima
    Shyamali, Narangoda Liyanage Ajantha
    Ogg, Graham Stuart
    Malavige, Gathsaurie Neelika
    BMC INFECTIOUS DISEASES, 2014, 14
  • [4] Performance of commercial dengue NS1 ELISA and molecular analysis of NS1 gene of dengue viruses obtained during surveillance in Indonesia
    Aryati, Aryati
    Trimarsanto, Hidayat
    Yohan, Benediktus
    Wardhani, Puspa
    Fahri, Sukmal
    Sasmono, R. Tedjo
    BMC INFECTIOUS DISEASES, 2013, 13
  • [5] Utility of dengue NS1 antigen rapid diagnostic test for use in difficult to reach areas and its comparison with dengue NS1 ELISA and qRT-PCR
    Shukla, Mohan K.
    Singh, Neeru
    Sharma, Ravendra K.
    Barde, Pradip V.
    JOURNAL OF MEDICAL VIROLOGY, 2017, 89 (07) : 1146 - 1150
  • [6] NS1 Antigen Testing for the Diagnosis of Dengue in Returned Israeli Travelers
    Fuchs, Inbal
    Bin, Hana
    Schlezinger, Sara
    Schwartz, Eli
    JOURNAL OF MEDICAL VIROLOGY, 2014, 86 (12) : 2005 - 2010
  • [7] Heterosandwich immunoswab assay for dengue virus Ns1 antigen detection
    Ganguly, Advaita
    Malabadi, Ravindra B.
    Loebenberg, Raimer
    Suresh, Mavanur R.
    Sunwoo, Hoon H.
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2014, 78 (01) : 35 - 39
  • [8] Detection of recombinant dengue virus 2 NS1 protein in Aedes aegypti mosquitoes using commercial Dengue NS1 ELISA kit
    Abraham, Philip
    Sankari, T.
    Kumar, N.
    Kumar, Ashwani
    JOURNAL OF VECTOR BORNE DISEASES, 2022, 59 (01) : 98 - 101
  • [9] Application of the Dengue Virus NS1 Antigen Rapid Test for On-Site Detection of Imported Dengue Cases at Airports
    Shu, Pei-Yun
    Yang, Cheng-Fen
    Kao, Jeng-Fong
    Su, Chien-Ling
    Chang, Shu-Fen
    Lin, Chien-Chou
    Yang, Wen-Chih
    Shih, Hsiu
    Yang, Shih-Yan
    Wu, Ping-Fuai
    Wu, Ho-Sheng
    Huang, Jyh-Hsiung
    CLINICAL AND VACCINE IMMUNOLOGY, 2009, 16 (04) : 589 - 591
  • [10] Dengue NS1 antigen kit shows high sensitivity for detection of recombinant dengue virus-2 NS1 antigen spiked with Aedes aegypti mosquitoes
    Abraham, Philip Raj
    Bharathy, R.
    Kumar N, Pradeep
    Kumar, Ashwani
    SCIENTIFIC REPORTS, 2021, 11 (01)
12345