The Efficacy of Composite Essential Oils against Aflatoxigenic Fungus Aspergillus flavus in Maize

被引：37
作者：
Xiang, Fangzhi [1 ]
Zhao, Qianqian [1 ]
Zhao, Kai [1 ]
Pei, Hao [1 ]
Tao, Fang [1 ]
机构：
[1] Anhui Agr Univ, Sch Life Sci, Hefei 230036, Peoples R China
来源：
TOXINS | 2020年 / 12卷 / 09期
关键词：
Aspergillus flavus; composite essential oils; synergistic effect; aflatoxin B1; maize grains; ANTIFUNGAL ACTIVITY; ANTIMICROBIAL ACTIVITY; OXIDATIVE STRESS; CHEMICAL-COMPOSITION; PROPIONIC-ACID; GROWTH; BIOSYNTHESIS; CONTAMINATION; ACCUMULATION; PARASITICUS;
D O I：
10.3390/toxins12090562
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The efficacy of eleven essential oils (EOs) against Aspergillus flavus NRRL 3357 was investigated. The highest antifungal activity against this aflatoxigenic fungus was exhibited by cinnamon, oregano and lemongrass, which showed low minimum inhibitory concentration (MIC) values under vapor conditions. Interactions of the three EOs were evaluated by the fractional inhibition concentration index (FICI), and the composite essential oils (CEO) showed synergistic inhibitory activities. Chemical analysis of the composite essential oils of cinnamon, oregano, and lemongrass (COL-CEO) revealed that (Z)-citral (33.44%), (E)-citral (32.88%) and carvacrol (19.84%) were the dominant components, followed by limonene (4.29%) and cinnamaldehyde (3.76%). COL-CEO not only inhibited fungal growth but also decreased aflatoxin B1 production by A. flavus. Downregulation of the relative expression of aflatoxin genes in the aflatoxin biosynthetic pathway by COL-CEO revealed its anti-aflatoxigenic mechanism. COL-CEO could also affect the colonization of A. flavus on maize grains. Therefore, COL-CEO may be considered as a potential natural antifungal agent, which could be used for the storage of maize and other grains.
引用
收藏
页数：16
相关论文
共 77 条
[11]   Antifungal activity and mechanism of citral, limonene and eugenol against Zygosaccharomyces rouxii [J].
Cai, Rui ;
Hu, Miaomiao ;
Zhang, Yijun ;
Niu, Chen ;
Yue, Tianli ;
Yuan, Yahong ;
Wang, Zhouli .
LWT-FOOD SCIENCE AND TECHNOLOGY, 2019, 106 :50-56
[12]  
Centers for Disease Control and Prevention (CDC), 2004, MMWR Morb Mortal Wkly Rep, V53, P790
[13]   Effect of peppermint oil on the shelf-life of dragon fruit during storage [J].
Chaemsanit, Siriporn ;
Matan, Narumol ;
Matan, Nirundorn .
FOOD CONTROL, 2018, 90 :172-179
[14]   Distribution of Aspergillus flavus and aflatoxin accumulation in stored maize grains across three agro-ecologies in Ghana [J].
Dadzie, M. A. ;
Oppong, A. ;
Ofori, K. ;
Eleblu, J. S. ;
Ifie, E. B. ;
Blay, E. ;
Obeng-Bio, E. ;
Appiah-Kubi, Z. ;
Warburton, M. L. .
FOOD CONTROL, 2019, 104 :91-98
[15]   Aflatoxin biosynthesis gene clusters and flanking regions [J].
Ehrlich, KC ;
Yu, J ;
Cotty, PJ .
JOURNAL OF APPLIED MICROBIOLOGY, 2005, 99 (03) :518-527
[16]   Essential oil composition and antifungal activity of Melissa officinalis originating from north-Est Morocco, against postharvest phytopathogenic fungi in apples [J].
El Ouadi, Y. ;
Manssouri, M. ;
Bouyanzer, A. ;
Majidi, L. ;
Bendaif, H. ;
Elmsellem, H. ;
Shariati, M. A. ;
Melhaoui, A. ;
Hammouti, B. .
MICROBIAL PATHOGENESIS, 2017, 107 :321-326
[17]   Chemical composition, antimicrobial activities and synergistic effects of essential oil from Senecio anteuphorbium, a Moroccan endemic plant [J].
Elhidar, Najoua ;
Nafis, Ahmed ;
Kasrati, Ayoub ;
Goehler, Andre ;
Bohnert, Juergen A. ;
Abbad, Abdelaziz ;
Hassani, Lahcen ;
Mezrioui, Nour-Eddine .
INDUSTRIAL CROPS AND PRODUCTS, 2019, 130 :310-315
[18]   Worldwide contamination of food-crops with mycotoxins: Validity of the widely cited 'FAO estimate' of 25% [J].
Eskola, Mari ;
Kos, Gregor ;
Elliott, Christopher T. ;
Hajslova, Jana ;
Mayar, Sultan ;
Krska, Rudolf .
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION, 2020, 60 (16) :2773-2789
[19]   Oxidative stress and carbon metabolism influence Aspergillus flavus transcriptome composition and secondary metabolite production [J].
Fountain, Jake C. ;
Bajaj, Prasad ;
Pandey, Manish ;
Nayak, Spurthi N. ;
Yang, Liming ;
Kumar, Vinay ;
Jayale, Ashwin S. ;
Chitikineni, Anu ;
Zhuang, Weijian ;
Scully, Brian T. ;
Lee, R. Dewey ;
Kemerait, Robert C. ;
Varshney, Rajeev K. ;
Guo, Baozhu .
SCIENTIFIC REPORTS, 2016, 6
[20]   A novel interpretation of the Fractional Inhibitory Concentration Index: The case Origanum vulgare L. and Leptospermum scoparium J. R. et G. Forst essential oils against Staphylococcus aureus strains [J].
Fratini, Filippo ;
Mancini, Simone ;
Turchi, Barbara ;
Friscia, Elisabetta ;
Pistelli, Luisa ;
Giusti, Giulia ;
Cerri, Domenico .
MICROBIOLOGICAL RESEARCH, 2017, 195 :11-17
12345678