Characterization of flavonoid glycosides from rapeseed bee pollen using a combination of chromatography, spectrometry and nuclear magnetic resonance with a step-wise separation strategy

被引：8

作者：

Li, Yi ^{[1
,2
,3
]}

Qi, Yitao ^{[4
]}

Ritho, Joan ^{[5
]}

Zhang, Yongxin ^{[6
]}

Zheng, Xiaowei ^{[7
]}

Zhou, Jinhui ^{[1
,2
,3
]}

Sun, Liping ^{[1
,8
]}

机构：

[1] Chinese Acad Agr Sci, Inst Apicultural Res, Beijing 100093, Peoples R China

[2] Minist Agr, Bee Prod Qual Supervis & Testing Ctr, Beijing 100093, Peoples R China

[3] Minist Agr, Lab Risk Assessment Qual & Safety Bee Prod, Beijing 100093, Peoples R China

[4] St Lukes Episcopal Hosp, Texas Heart Inst, Houston, TX 77030 USA

[5] Univ Texas MD Anderson Canc Ctr, Dept Cardiol, Houston, TX 77030 USA

[6] China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 10070, Peoples R China

[7] Henan Univ, Sch Med, Henan Engn Lab Antibody Med, Kaifeng 475001, Peoples R China

[8] Minist Agr, Key Lab Pollinating Insect Biol, Beijing 100093, Peoples R China

来源：

NATURAL PRODUCT RESEARCH | 2016年 / 30卷 / 02期

基金：

中国国家自然科学基金;

关键词：

bee pollen; step-wise separation strategy; semi-preparative HPLC; LC-DAD-ESI-MS; NMR; flavonoid glycosides; OLIGOLYSINE;

D O I：

10.1080/14786419.2015.1041942

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

To identify the structures of flavonoid glycosides in bee pollen collected from rapeseed plants (Brassica napus L.), we utilised an approach that combined liquid chromatography-diode array detector-electrospray ionization-mass spectrometry (LC-DAD-ESI-MS) and nuclear magnetic resonance (NMR) technology with a step-wise separation strategy. We identified four constituents of high purity in rape bee pollen samples: (1) quercetin-3-O--D-glucosyl-(2l)--glucoside, (2) kaempferol-3, 4-di-O--D-glucoside, (3) 5, 7, 4-trihydroxy-3-methoxyflavone-3-O--D-sophoroside and (4) kaempferol-3-O--D-glucosyl-(2l)--D-glucoside. This study will also provide useful reference standards for qualification and quantification of four flavonoid glycosides in natural products.

引用

页码：228 / 231

页数：4

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