Effect of serum withdrawal on the proliferation of B16F10 melanoma cells

被引：9
|
作者：
Rodriguez-Ayerbe, C [1 ]
Smith-Zubiaga, I [1 ]
机构：
[1] Univ Basque Country, Fac Sci, Dept Zool & Anim Cell Dynam, Bilbao 48080, Spain
来源：
CELL BIOLOGY INTERNATIONAL | 2000年 / 24卷 / 05期
关键词：
B16F10 melanoma cells; serum deprivation; PCNA; BrdU; flow cytometry; cell cycle;
D O I：
10.1006/cbir.1999.0502
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
B16F10 murine melanoma cell proliferation was inhibited after 48 h in medium with serum in the range 0.1 to 0.5% by volume. Cell viability was mostly retained, whereas cells completely deprived of serum died. Growth-arrested cultures showed serum-dependent suppression of DNA synthesis. The response was typically that of a 'cell cycle freeze', verified by flow cytometric distribution of cells. Consequently, serum deprivation did not lead to synchony when serum was restored to arrested populations. Furthermore, there was no change in PCNA expression in arrested cells. (C) 2000 Academic Press.
引用
收藏
页码：279 / 283
页数：5
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