Pancreatic beta cell function persists in many patients with chronic type 1 diabetes, but is not dramatically improved by prolonged immunosuppression and euglycaemia from a beta cell allograft

被引：43

作者：

Liu, E. H. ^{[1
]}

Digon, B. J., III ^{[1
]}

Hirshberg, B. ^{[1
]}

Chang, R. ^{[2
]}

Wood, B. J. ^{[2
]}

Neeman, Z. ^{[2
]}

Kam, A. ^{[2
]}

Wesley, R. A. ^{[3
]}

Polly, S. M. ^{[4
]}

Hofmann, R. M. ^{[5
]}

Rother, K. I. ^{[1
]}

Harlan, D. M. ^{[1
]}

机构：

[1] NIDDKD, Diabet Branch, NIH, Bethesda, MD 20892 USA

[2] NIH, Ctr Clin, Dept Diagnost Radiol, Bethesda, MD 20892 USA

[3] NIH, Ctr Clin, Off Director, Bethesda, MD 20892 USA

[4] Walter Reed Army Med Ctr, Washington, DC 20307 USA

[5] Univ Wisconsin, Dept Med, Madison, WI USA

来源：

DIABETOLOGIA | 2009年 / 52卷 / 07期

关键词：

Beta cell regeneration; C-peptide; Islet transplantation; Pancreas transplantation; Type; 1; diabetes; T-CELLS; MELLITUS; REGENERATION; HUMANS; GROWTH; AUTOIMMUNITY; ANTIBODIES; TURNOVER; ADULT; ONSET;

D O I：

10.1007/s00125-009-1342-7

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We measured serum C-peptide (at least 0.167 nmol/l) in 54 of 141 (38%) patients with chronic type 1 diabetes and sought factors that might differentiate those with detectable C-peptide from those without it. Finding no differences, and in view of the persistent anti-beta cell autoimmunity in such patients, we speculated that the immunosuppression (to weaken autoimmune attack) and euglycaemia accompanying transplant-based treatments of type 1 diabetes might promote recovery of native pancreatic beta cell function. We performed arginine stimulation tests in three islet transplant and four whole-pancreas transplant recipients, and measured stimulated C-peptide in select venous sampling sites. On the basis of each sampling site's C-peptide concentration and kinetics, we differentiated insulin secreted from the individual's native pancreatic beta cells and that secreted from allografted beta cells. Selective venous sampling demonstrated that despite long-standing type 1 diabetes, all seven beta cell allograft recipients displayed evidence that their native pancreas secreted C-peptide. Yet even if chronic immunosuppression coupled with near normal glycaemia did improve native pancreatic C-peptide production, the magnitude of the effect was quite small. Some native pancreatic beta cell function persists even years after disease onset in most type 1 diabetic patients. However, if prolonged euglycaemia plus anti-rejection immunosuppressive therapy improves native pancreatic insulin production, the effect in our participants was small. We may have underestimated pancreatic regenerative capacity by studying only a limited number of participants or by creating conditions (e.g. high circulating insulin concentrations or immunosuppressive agents toxic to beta cells) that impair beta cell function. ClinicalTrials.gov NCT00246844 and NCT00006505.

引用

页码：1369 / 1380

页数：12