In vitro activity of azole derivatives and griseofulvin against planktonic and biofilm growth of clinical isolates of dermatophytes

被引：18

作者：

Nogueira Brilhante, Raimunda Samia ^{[1
]}

Monteiro Correia, Edmilson Emanuel ^{[1
]}

de Melo Guedes, Glaucia Morgana ^{[1
]}

de Oliveira, Jonathas Sales ^{[1
]}

Collares Maia Castelo-Branco, Debora de Souza ^{[1
]}

Cordeiro, Rossana de Aguiar ^{[1
]}

Pinheiro, Adriana de Queiroz ^{[2
]}

Queiroz Chaves, Lucio Jackson ^{[1
]}

Pereira Neto, Waldemiro de Aquino ^{[1
]}

Costa Sidrim, Jose Julio ^{[1
]}

Gadelha Rocha, Marcos Fabio ^{[1
,2
]}

机构：

[1] Univ Fed Ceara, Dept Pathol & Legal Med, Postgrad Program Med Microbiol, Specialized Med Mycol Ctr,Sch Med, Fortaleza, Ceara, Brazil

[2] Univ Estadual Ceara, Sch Vet, Postgrad Program Vet Sci, Fortaleza, Ceara, Brazil

来源：

MYCOSES | 2018年 / 61卷 / 07期

关键词：

biofilm; dermatophytes; human isolates; planktonic; susceptibility testing; veterinary isolates; TRICHOPHYTON-RUBRUM; ANTIFUNGAL AGENTS; RESISTANCE; SUSCEPTIBILITY; EPIDEMIOLOGY;

D O I：

10.1111/myc.12763

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

As shown by recent research, most of the clinically relevant fungi, including dermatophytes, form biofilms in vitro and in vivo, which may exhibit antimicrobial tolerance that favour recurrent infections. The aim of this study was to determine the minimum inhibitory concentrations (MICs) of itraconazole (ITC), voriconazole (VCZ) and griseofulvin (GRI) against Trichophyton rubrum, Trichophyton tonsurans, Trichophyton mentagrophytes, Microsporum canis and Microsporum gypseum in planktonic and biofilm growth. For the planktonic form, susceptibility testing was performed according to the Clinical and Laboratory Standards Institute (CLSI), document M38-A2, while biofilm susceptibility was evaluated using the XTT colorimetric essay. The planktonic growth of all strains was inhibited, with MIC values ranging from 0.00195 to 0.1225g/mL for VRC, 0.00195 to 0.25g/mL for ITC and <0.0039 to 4g/mL for GRI, while a 50-fold increase in the MIC was required to significantly reduce the metabolic activity (P<.05) of dermatophyte biofilms. In brief, the ability of dermatophytes to form biofilms may be a contributing factor for the recalcitrance of dermatophytoses or the dissemination of the disease.

引用

页码：449 / 454

页数：6

共 34 条

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