Enzymatic characterization of the major phospholipase A2 component of sea anemone (Aiptasia pallida) nematocyst venom

被引:26
作者
Grotendorst, GR
Hessinger, DA [1 ]
机构
[1] Loma Linda Univ, Sch Med, Dept Physiol & Pharmacol, Loma Linda, CA 92350 USA
[2] Univ Miami, Sch Med, Dept Cell Biol & Anat, Miami, FL 33136 USA
关键词
phospholipase A(2); toxin; sea anemone; Aiptasia pallida; nematocyst; hemolysis;
D O I
10.1016/S0041-0101(99)00206-8
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The purified beta phospholipase A(2) (PLA(2); EC 3.1.1.4) (PLA(2)) from sea anemone (Aiptasia pallida) nematocysts is larger and more labile than other known venom PLA(2)s. In common with all other known venoms and most secretory PLA(2)s, the beta PLA(2) requires mM Ca2+ for optimal activity and is surface-activated by aggregated lipids such as mixed micelles of detergent and phospholipid. The beta PLA(2) exhibits an unusually steep and narrow pH optimum of activity at pH 7.7. The effects of changes in pH on the activity of the enzyme suggest that the active site contains functional groups having a pK's of about 7.0 and 8.0, The effects of temperature on beta PLA(2) activity show a marked decrease in the energy of activation above the pre-transition temperature, suggesting that the enzyme "melts" both fatty chains in order for catalysis to occur. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:931 / 943
页数:13
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