Simultaneous analysis of cocaine and its metabolites in rat liver microsome s9 fraction by liquid chromatography-mass spectrometry

A method utilizing liquid chromatography-mass spectrometry for the simultaneous analysis of cocaine and its in-vitro metabolites in rat liver micro some S-9 fraction is described. Cocaine was incubated with rat liver micro some S-9 fraction firstly, and then the incubation sample mixture was analyzed by liquid chromatography-mass spectrometry in full-scan mode. The main metabolites of cocaine were identified from the determination of molecular ions and the results of tandem mass spectrometry analysis. There were two metabolites were identified in the rat liver S-9 fractions referring to the literature reports.