Microbial uptake of free cathodic electrons presents a poorly understood aspect of microbial physiology. Uptake of cathodic electrons is particularly important in microbial electrosynthesis of sustainable fuel and chemical precursors using only CO2 and electricity as carbon, electron and energy source. Typically, large overpotentials (200 to 400 mV) were reported to be required for cathodic electron uptake during electrosynthesis of, for example, methane and acetate, or low electrosynthesis rates were observed. To address these limitations and to explore conceptual alternatives, we studied defined co-cultures metabolizing cathodic electrons. The Fe(0)-corroding strain IS4 was used to catalyze the electron uptake reaction from the cathode forming molecular hydrogen as intermediate, and Methanococcus maripaludis and Acetobacterium woodii were used as model microorganisms for hydrogenotrophic synthesis of methane and acetate, respectively. The IS4-M. maripaludis co-cultures achieved electromethanogenesis rates of 0.1-0.14 mu mol cm(-2) h(-1) at - 400 mV vs standard hydrogen electrode and 0.6-0.9 mu mol cm(-2) h(-1) at -500 mV. Co-cultures of strain IS4 and A. woodii formed acetate at rates of 0.21-0.23 mu mol cm(-2) h(-1) at -400 mV and 0.57-0.74 mu mol cm(-2) h(-1) at -500 mV. These data show that defined co-cultures coupling cathodic electron uptake with synthesis reactions via interspecies hydrogen transfer may lay the foundation for an engineering strategy for microbial electrosynthesis.
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Univ Roma La Sapienza, Dept Chem, I-00185 Rome, Italy
Natl Res Council CNR IRSA, Water Res Inst, I-00015 Monterotondo, RM, ItalyUniv Roma La Sapienza, Dept Chem, I-00185 Rome, Italy
Aulenta, Federico
Catapano, Laura
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Univ Roma La Sapienza, Dept Chem, I-00185 Rome, ItalyUniv Roma La Sapienza, Dept Chem, I-00185 Rome, Italy
Catapano, Laura
Snip, Laura
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Univ Roma La Sapienza, Dept Chem, I-00185 Rome, Italy
Wageningen Univ, Subdept Environm Technol, NL-6700 AA Wageningen, NetherlandsUniv Roma La Sapienza, Dept Chem, I-00185 Rome, Italy
Snip, Laura
Villano, Marianna
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Univ Roma La Sapienza, Dept Chem, I-00185 Rome, ItalyUniv Roma La Sapienza, Dept Chem, I-00185 Rome, Italy
Villano, Marianna
Majone, Mauro
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Univ Roma La Sapienza, Dept Chem, I-00185 Rome, ItalyUniv Roma La Sapienza, Dept Chem, I-00185 Rome, Italy
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Univ Roma La Sapienza, Dept Chem, I-00185 Rome, Italy
Natl Res Council CNR IRSA, Water Res Inst, I-00015 Monterotondo, RM, ItalyUniv Roma La Sapienza, Dept Chem, I-00185 Rome, Italy
Aulenta, Federico
Catapano, Laura
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Univ Roma La Sapienza, Dept Chem, I-00185 Rome, ItalyUniv Roma La Sapienza, Dept Chem, I-00185 Rome, Italy
Catapano, Laura
Snip, Laura
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Univ Roma La Sapienza, Dept Chem, I-00185 Rome, Italy
Wageningen Univ, Subdept Environm Technol, NL-6700 AA Wageningen, NetherlandsUniv Roma La Sapienza, Dept Chem, I-00185 Rome, Italy
Snip, Laura
Villano, Marianna
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h-index: 0
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Univ Roma La Sapienza, Dept Chem, I-00185 Rome, ItalyUniv Roma La Sapienza, Dept Chem, I-00185 Rome, Italy
Villano, Marianna
Majone, Mauro
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Univ Roma La Sapienza, Dept Chem, I-00185 Rome, ItalyUniv Roma La Sapienza, Dept Chem, I-00185 Rome, Italy