Measurements of behavioral quiescence in Caenorhabditis elegans

被引:33
作者
Nagy, Stanislav [1 ]
Raizen, David M. [2 ]
Biron, David [1 ,3 ]
机构
[1] Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA
[2] Univ Penn, Pereleman Sch Med, Dept Neurol, Philadelphia, PA 19104 USA
[3] Univ Chicago, James Franck Inst, Dept Phys, Chicago, IL 60637 USA
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
Quiescence; Elegans; Sleep; Molting; Lethargus; Machine vision; SLEEP-LIKE STATE; C-ELEGANS; CIRCUIT; DROSOPHILA; LETHARGUS; NEURONS; SYSTEM; CGMP;
D O I
10.1016/j.ymeth.2014.03.009
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The nematode Caenorhabditis (C.) elegans, a long time work horse for behavioral genetic studies of locomotion, has recently been studied for quiescent behavior. Methods previously established for the study of C elegans locomotion are not well-suited for the study of quiescent behavior. We describe in detail two computer vision approaches to distinguish quiescent from movement bouts focusing on the behavioral quiescence that occurs during fourth larval stage lethargus, a transition stage between the larva and the adult. The first is the frame subtraction method, which consists of subtraction of temporally adjacent images as a sensitive way to detect motion. The second, which is more computationally intensive, is the posture analysis method, which consists of analysis of the rate of local angle change of the animal's body. Quiescence measurements should be done continuously while minimizing sensory perturbation of the animal. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码:500 / 507
页数:8
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