A quantitative real-time PCR method for absolute telomere length

被引：184

作者：

O'Callaghan, Nathan J. ^{[1
]}

Dhillon, Varinderpal S. ^{[1
]}

Thomas, Philip ^{[1
]}

Fenech, Michael ^{[1
]}

机构：

[1] CSIRO Human Nutr, Adelaide, SA 5000, Australia

来源：

BIOTECHNIQUES | 2008年 / 44卷 / 06期

关键词：

D O I：

10.2144/000112761

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Telomere shortening is tin important risk factor for cancer and accelerated aging, Here we describe the development of a simple and reproducible method to measure absolute telomere length. Based on Cawthon's quantitative real-time PCR (qRT-PCR) assay, our method uses an oligomer standard that can be used to generate absolute telomere length values rather than relative quantification. Me demonstrate a strong correlation between this improved method and the "gold standard" of telomere length measurement-terminal restriction fragment analysis (TRF) by, Southern hybridization. The capability to generate absolute telomere length values should allow a more direct comparison of results between experiments within and between laboratories.

引用

页码：807 / 809

页数：3

共 17 条

[1] A method for detecting abasic sites in living cells: Age-dependent changes in base excision repair [J].