Preparation of Three-Dimensional Vascularized MSC Cell Sheet Constructs for Tissue Regeneration

被引:48
作者
Ren, Liling [1 ]
Ma, Dongyang [2 ]
Liu, Bin [1 ]
Li, Jinda [1 ]
Chen, Jia [1 ]
Yang, Dan [1 ]
Gao, Peng [1 ]
机构
[1] Lanzhou Univ, Sch Stomatol, Lanzhou 730000, Gansu, Peoples R China
[2] Lanzhou Command PLA, Lanzhou Gen Hosp, Dept Oral & Maxillofacial Surg, Lanzhou 730050, Gansu, Peoples R China
基金
中国国家自然科学基金;
关键词
MOLECULAR REGULATION; ENDOTHELIAL-CELLS; STEM-CELLS; SCAFFOLDS; TRANSPLANTATION; MORPHOGENESIS; ANGIOGENESIS;
D O I
10.1155/2014/301279
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Engineering three-dimensional (3D) vascularized constructs remains a challenge due to the inability to form rich microvessel networks. In this study we engineered a prevascularized 3D cell sheet construct for tissue regeneration using human bone marrow-derived mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells as cell sources. hMSCs were cultured to forma thick cell sheet, and human umbilical vein endothelial cells (HUVECs) were then seeded on the hMSCs sheet to form networks. The single prevascularized HUVEC/hMSC cell sheet was folded to forma 3D construct by a modified cell sheet engineering technique. In vitro results indicated that the hMSCs cell sheet promoted the HUVECs cell migration to form networks in horizontal and vertical directions. In vivo results showed that many blood vessels grew into the 3D HUVEC/hMSC cell sheet constructs after implanted in the subcutaneous pocket of immunodeficient mice. The density of blood vessels in the prevascularized constructs was higher than that in the nonprevascularized constructs. Immunohistochemistry staining further showed that in vitro preformed human capillaries in the prevascularized constructs anastomosed with the host vasculature to form functional blood vessels. These results suggest the promising potential of this 3D prevascularized construct using hMSCs cell sheet as a platform for wide applications in engineering vascularized tissues.
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页数:10
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