Lentivirus Vectors for Stably Introducing Genes into Mammary Epithelial Cells in Vivo

被引：33

作者：

Bu, Wen ^{[2
]}

Xin, Li ^{[1
,3
]}

Toneff, Michael ^{[1
,2
]}

Li, Lei ^{[4
]}

Li, Yi ^{[1
,2
]}

机构：

[1] Baylor Coll Med, Dept Mol & Cell Biol, Houston, TX 77030 USA

[2] Baylor Coll Med, Lester & Sue Smith Breast Ctr, Houston, TX 77030 USA

[3] Baylor Coll Med, Dept Pathol, Houston, TX 77030 USA

[4] Univ Texas MD Anderson Canc Ctr, Dept Expt Radiat Oncol, Dept Genet, Houston, TX 77030 USA

来源：

JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA | 2009年 / 14卷 / 04期

关键词：

Lentivirus; Mammary gland; Breast cancer; Somatic cell; TVA; RCAS;

D O I：

10.1007/s10911-009-9154-4

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We have reported the use of vectors based on avian leukosis virus (ALV) and ALV envpseudotyped lentivirus for stably introducing genes into somatic mammary cells in vivo for tumor modeling. These vectors require tva transgenic mice for viral infection. Here, we report, and described the method for, stably introducing genes into the mammary gland in mice using intraductal injection of a common lentiviral vector. This method does not need tva-transgenic mice for infection. This in vivo transformation method may be useful for rapid testing of the tumorigenic potential of genes potentially involved in breast carcinogenesis.

引用

页码：401 / 404

页数：4

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