Isolation of a lectin and a galactoxyloglucan from Mucuna sloanei seeds

被引：9

作者：

Teixeira-Sa, Daniele M. A. ^{[1
,2
]}

Reicher, Fany ^{[3
]}

Braga, Renata C. ^{[2
]}

Beltramini, Leila Maria ^{[4
]}

Moreira, Renato de Azevedo ^{[2
,5
]}

机构：

[1] UECE, Curo Quim, Fac Filosofia Dom Aureliano Matos, Lab Quim, BR-62930000 Limoeiro Do Norte, Ceara, Brazil

[2] Univ Fed Ceara, Dept Bioquim & Biol Mol, Lab Lectinas & Glicoconjugados, BR-60451980 Fortaleza, Ceara, Brazil

[3] Univ Fed Parana, Dept Bioquim & Biol Mol, BR-81531970 Curitiba, Parana, Brazil

[4] Univ Sao Paulo, Inst Fis Sao Carlos, Dept Fis & Informat, Sao Carlos, SP, Brazil

[5] Univ Fortaleza, Ctr Ciencias Saude, BR-60811905 Fortaleza, Ceara, Brazil

来源：

PHYTOCHEMISTRY | 2009年 / 70卷 / 17-18期

关键词：

Mucuna sloanei; Fabaceae; Galactoxyloglucan; Lectin; Sloanin; POLYACRYLAMIDE-GEL; BINDING-LECTIN; CELL-WALL; PROTEIN; POLYSACCHARIDE; XYLOGLUCAN; PRURIENS; GUM; CONCANAVALIN; BIOCHEMISTRY;

D O I：

10.1016/j.phytochem.2009.08.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A lectin and a galactoxyloglucan were characterized from Mucuna sloanei seed cotyledons. The galactoxyloglucan, isolated by water extraction and ethanol precipitation, had Glc:Xyl:Gal proportions in a molar ratio of 1.8:1.7:1.0 and a molar mass (M-w) of 1.6 x 10(6) g mol(-1). The lectin (sloanin), isolated from the same seed by affinity chromatography on cross-linked Adenanthera pavonina galactomannan, gave two protein bands by SDS-PAGE (36 and 34 kDa) and one peak by gel filtration (63.6 kDa). Its N-terminal sequence indicated similar to 69% identity with soybean agglutinin to leguminous lectins. Circular dichroism (CD) spectra established that sloanin predominantly contains beta-sheet structures. Sloanin has similar to 5.5% carbohydrate and displayed hemagglutinating activity against rabbit and enzyme treated human erythrocytes, inhibited only by D-Gal containing sugars. The interaction between sloanin and storage cell-wall galactoxyloglucan was tested by affinity chromatography and fluorescence spectroscopy. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1965 / 1972

页数：8

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