Isolation of a lectin and a galactoxyloglucan from Mucuna sloanei seeds

被引:9
作者
Teixeira-Sa, Daniele M. A. [1 ,2 ]
Reicher, Fany [3 ]
Braga, Renata C. [2 ]
Beltramini, Leila Maria [4 ]
Moreira, Renato de Azevedo [2 ,5 ]
机构
[1] UECE, Curo Quim, Fac Filosofia Dom Aureliano Matos, Lab Quim, BR-62930000 Limoeiro Do Norte, Ceara, Brazil
[2] Univ Fed Ceara, Dept Bioquim & Biol Mol, Lab Lectinas & Glicoconjugados, BR-60451980 Fortaleza, Ceara, Brazil
[3] Univ Fed Parana, Dept Bioquim & Biol Mol, BR-81531970 Curitiba, Parana, Brazil
[4] Univ Sao Paulo, Inst Fis Sao Carlos, Dept Fis & Informat, Sao Carlos, SP, Brazil
[5] Univ Fortaleza, Ctr Ciencias Saude, BR-60811905 Fortaleza, Ceara, Brazil
关键词
Mucuna sloanei; Fabaceae; Galactoxyloglucan; Lectin; Sloanin; POLYACRYLAMIDE-GEL; BINDING-LECTIN; CELL-WALL; PROTEIN; POLYSACCHARIDE; XYLOGLUCAN; PRURIENS; GUM; CONCANAVALIN; BIOCHEMISTRY;
D O I
10.1016/j.phytochem.2009.08.011
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A lectin and a galactoxyloglucan were characterized from Mucuna sloanei seed cotyledons. The galactoxyloglucan, isolated by water extraction and ethanol precipitation, had Glc:Xyl:Gal proportions in a molar ratio of 1.8:1.7:1.0 and a molar mass (M-w) of 1.6 x 10(6) g mol(-1). The lectin (sloanin), isolated from the same seed by affinity chromatography on cross-linked Adenanthera pavonina galactomannan, gave two protein bands by SDS-PAGE (36 and 34 kDa) and one peak by gel filtration (63.6 kDa). Its N-terminal sequence indicated similar to 69% identity with soybean agglutinin to leguminous lectins. Circular dichroism (CD) spectra established that sloanin predominantly contains beta-sheet structures. Sloanin has similar to 5.5% carbohydrate and displayed hemagglutinating activity against rabbit and enzyme treated human erythrocytes, inhibited only by D-Gal containing sugars. The interaction between sloanin and storage cell-wall galactoxyloglucan was tested by affinity chromatography and fluorescence spectroscopy. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1965 / 1972
页数:8
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