Scopulibacillus darangshiensis gen. nov., sp nov., Isolated from Rock

A novel, Gram-positive bacterium, designated DLS-06(T), was isolated from scoria (volcanic ash) under rock on the peak of small mountain (300 m above the sea level; known as Darangshi Oreum) in Jeju, Republic of Korea. The cells of the isolate were aerobic, oxidase-negative, catalase-positive, endosporeforming, non-motile rods. The organism grew at 25 similar to 30 degrees C and initial pH 6.1 similar to 9.1. A neighbour-joining tree based on 16S rRNA gene sequences showed that the organism was related to members of the family "Sporolactobacillaceae" and related taxa. The phylogenetic neighbours were Pullulanibacillus naganoensis (95.2% 16S rRNA gene sequence similarity), Tuberibacillus calidus (95.0%) and Sporolactobacillus (91.8 similar to 94.2%). Levels of 16S rRNA gene sequence similarity of the isolate to representatives of other genera were in the range or 87.2 similar to 93.7%. The organism contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The predominant menaquinone was MK-7. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, an unknown ninhydrin-positive phospholipid, three unknown phospholipids and an unknown lipid. The major fatty acids were anteiso-C(15:0) and anteiso-C(17:0). The G+C content of the DNA was 50.8 mol%. On the basis of the phenotypic and phylogenetic data presented in this study, this organism represents a novel genus and species in the order Bacillales, for which the name Scopulibacillus darangshiensis gen. nov., sp. nov. is proposed. The type strain is DLS-06(T) (=DSM 19377(T) = KCTC 13161(T))