A slicer-mediated mechanism for repeat-associated siRNA 5′ end formation in Drosophila

被引：915

作者：

Gunawardane, Lalith S. ^{[1
]}

Saito, Kuniaki ^{[1
]}

Nishida, Kazumichi M. ^{[1
]}

Miyoshi, Keita ^{[1
]}

Kawamura, Yoshinori ^{[1
]}

Nagami, Tomoko ^{[1
]}

Siomi, Haruhiko ^{[1
]}

Siomi, Mikiko C. ^{[1
]}

机构：

[1] Univ Tokushima, Inst Genome Res, Tokushima 7708503, Japan

来源：

SCIENCE | 2007年 / 315卷 / 5818期

关键词：

D O I：

10.1126/science.1140494

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In Drosophila, repeat-associated small interfering RNAs (rasiRNAs) are produced in the germ line by a Dicer-independent pathway and function through the PIWI subfamily of Argonautes to ensure silencing of retrotransposons. We sequenced small RNAs associated with the PIWI subfamily member AGO3. Although other members of PIWI, Aubergine (Aub) and Piwi, associated with rasiRNAs derived mainly from the antisense strand of retrotransposons, AGO3-associated rasiRNAs arose mainly from the sense strand. Aub- and Piwi-associated rasiRNAs showed a strong preference for uracil at their 5' ends, and AGO3-associated rasiRNAs showed a strong preference for adenine at nucleotide 10. Comparisons between AGO3- and Aub- associated rasiRNAs revealed pairs of rasiRNAs showing complementarities in their first 10 nucleotides. Aub and AGO3 exhibited Slicer activity in vitro. These data support a model in which formation of a 5' terminus within rasiRNA precursors is guided by rasiRNAs originating from transcripts of the other strand in concert with the Slicer activity of PIWI.

引用

页码：1587 / 1590

页数：4

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