Analysis of adaptation to high ethanol concentration in Saccharomyces cerevisiae using DNA microarray

被引：35

作者：

Dinh, Thai Nho ^{[1
]}

Nagahisa, Keisuke ^{[2
]}

Yoshikawa, Katsunori ^{[2
]}

Hirasawa, Takashi ^{[2
]}

Furusawa, Chikara ^{[2
]}

Shimizu, Hiroshi ^{[2
]}

机构：

[1] Osaka Univ, Grad Sch Engn, Dept Biotechnol, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Grad Sch Informat Sci & Technol, Dept Bioinformat Engn, Suita, Osaka 5650871, Japan

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2009年 / 32卷 / 05期

关键词：

Adaptation; Ethanol; Saccharomyces cerevisiae; DNA microarray; STRESS-RESPONSE; GENE-EXPRESSION; YEAST TRANSCRIPTOME; STATIONARY-PHASE; SAKE YEAST; TOLERANCE; STRAINS; IDENTIFICATION; FERMENTATION; GROWTH;

D O I：

10.1007/s00449-008-0292-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In industrial process, yeast cells are exposed to ethanol stress that affects the cell growth and the productivity. Thus, investigating the intracellular state of yeast cells under high ethanol concentration is important. In this study, using DNA microarray analysis, we performed comprehensive expression profiling of two strains of Saccharomyces cerevisiae, i.e., the ethanol-adapted strain that shows active growth under the ethanol stress condition and its parental strain used as the control. By comparing the expression profiles of these two strains under the ethanol stress condition, we found that the genes related to ribosomal proteins were highly up-regulated in the ethanol-adapted strain. Further, genes related to ATP synthesis in mitochondria were suggested to be important for growth under ethanol stress. We expect that the results will provide a better understanding of ethanol tolerance of yeast.

引用

页码：681 / 688

页数：8

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