Flavobacterium lacunae sp nov., isolated from a freshwater pond

A novel bacterial strain AHQ-46(T) was isolated from a freshwater lake in Taiwan. The strain was Gram-stain-negative, strictly aerobic, motile by gliding, rod-shaped and formed translucent yellow colonies. Optimal growth occurred at 20-25 degrees C, pH 7.0, and in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain AHQ-46(T) belonged to the genus Flavobacterium and was most closely related to Flavobacterium verecundum TTM-46(T) with a sequence similarity of 95.8%. Strain AHQ-46(T) contained iso-C-15:1 G, iso-C-15:0, summed feature 3 (C-16:1 omega 6c and/or C-16:1 omega 7c) and iso-C-17:0 3-OH as the predominant fatty acids. The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized aminophospholipids and phospholipids. The major polyamine was homospermidine. The major isoprenoid quinone was MK-6. The DNA G+C content of the genomic DNA was 32.1 mol%. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain AHQ-46(T) should be classified as a representative of a novel species of the genus Flavobacterium, for which the name Flavobacterium lacunae sp. nov. is proposed. The type strain is AHQ-46(T) (=BCRC 80889(T) = LMG 28710(T)).