Identification, occurrence, incidence and transmission of phytoplasma associated with Petunia violacea witches' broom in Iran

被引:5
作者
Hemmati, Chamran [1 ,2 ]
Nikooei, Mehrnoosh [1 ]
Bertaccini, Assunta [3 ]
机构
[1] Univ Hormozgan, Minab Higher Educ Ctr, Dept Agr, Bandar Abbas, Iran
[2] Univ Hormozgan, Plant Protect Res Grp, Bandar Abbas, Iran
[3] Univ Bologna, Dept Agr & Food Sci, Alma Mater Studiorum, Bologna, Italy
关键词
16SrII-D; molecular identification; Orosius albicinctus; phytoplasma; purple petunia; 16S RIBOSOMAL-RNA; OROSIUS-ALBICINCTUS; HYBRIDA CULTIVARS; INSECT VECTOR; DISEASE; PHYLLODY;
D O I
10.1111/jph.12838
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A petunia witches' broom (PvWB) disease, characterized by phyllody, virescence, witches' broom, little leaf and yellowing, was observed in municipal lands and parks in Bandar Abbas, Hormozgan province, Iran. The disease was present with an average incidence of 20%. PCR and sequencing analysis carried out on selected samples from symptomatic plants showed the presence of a phytoplasma associated with the disease. The molecular comparison of the 16S ribosomal gene indicated 99% sequence identity with the one of "Candidatus Phytoplasma australasia". This phytoplasma was transmitted to healthy petunia plants under experimental conditions by the leafhopper Orosius albicinctus that was then demonstrated to be a vector of this phytoplasma.
引用
收藏
页码:547 / 552
页数:6
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