Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation

被引：50

作者：

Elkord, Eyad ^{[1
]}

机构：

[1] Univ Manchester, Dept Med Oncol, Clin Immunotherapy Lab, Manchester M20 4BX, Lancs, England

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2009年 / 347卷 / 1-2期

关键词：

T regulatory cell; Cryopreservation; INCREASED POPULATIONS; CARCINOMA; FOXP3;

D O I：

10.1016/j.jim.2009.06.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：87 / 90

页数：4

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