Integrated mRNA and microRNA transcriptome analyses provide insights into paclobutrazol inhibition of lateral branching in herbaceous peony

Herbaceous peony (Paeonia lactiflora Pall.) is a new high-end cut flower, but a large number of lateral branches often appear in some excellent cultivars, which is inconvenient for cut flower production. In the present study, we analyzed the effects of paclobutrazol (PBZ) on the lateral branches of P. lactiflora and adopted a next-generation sequencing approach to identify miRNAs and mRNAs that were differentially expressed involved in the PBZ response. Our results indicate that PBZ may inhibit the production of lateral branches on P. lactiflora. There were 827 differentially expressed genes (DEGs) and 104 differentially expressed miRNAs (DEMs). Integrative analysis revealed 29 miRNA-mRNA interactions related to PBZ stress. Our results provided a wealth of genetic information and data on metabolic pathways for revealing the regulatory mechanism of PBZ inhibition of the development of lateral branches in P. lactiflora and provided a new possibility for reducing lateral branch formation in the production of herbaceous peony cut flowers.