High-speed axial-scanning wide-field microscopy for volumetric particle tracking velocimetry

被引:13
作者
Chen, T. -H. [1 ]
Ault, J. T. [1 ]
Stone, H. A. [1 ]
Arnold, C. B. [1 ]
机构
[1] Princeton Univ, Dept Mech & Aerosp Engn, Princeton, NJ 08544 USA
关键词
ACOUSTIC GRADIENT-INDEX; IMAGE VELOCIMETRY; T-JUNCTION; FLOWS; LENS;
D O I
10.1007/s00348-017-2316-z
中图分类号
TH [机械、仪表工业];
学科分类号
0802 ;
摘要
The ability to understand and visualize complex flow structures in microfluidic and biological systems relies heavily on the resolving power of three-dimensional (3D) particle velocimetry techniques. We propose a simple technique for acquiring volumetric particle information with the potential for microsecond time resolution. By utilizing a fast varifocal lens in a modified wide-field microscope, we capture both volumetric and planar information with microsecond time resolution. The technique is demonstrated by tracking particle motions in the complex, three-dimensional flow in a high Reynolds number laminar flow at a branching arrow-shaped junction.
引用
收藏
页数:7
相关论文
共 27 条
[11]   An improved three-dimensional characterization of defocusing digital particle image velocimetry (DDPIV) based on a new imaging volume definition [J].
Grothe, R. L. ;
Dabiri, D. .
MEASUREMENT SCIENCE AND TECHNOLOGY, 2008, 19 (06)
[12]   Three-dimensional three-component particle velocimetry for microscale flows using volumetric scanning [J].
Klein, S. A. ;
Moran, J. L. ;
Frakes, D. H. ;
Posner, J. D. .
MEASUREMENT SCIENCE AND TECHNOLOGY, 2012, 23 (08)
[13]   Mechanics and refractive power optimization of tunable acoustic gradient lenses [J].
McLeod, Euan ;
Arnold, Craig B. .
JOURNAL OF APPLIED PHYSICS, 2007, 102 (03)
[14]   High-speed varifocal imaging with a tunable acoustic gradient index of refraction lens [J].
Mermillod-Blondin, Alexandre ;
McLeod, Euan ;
Arnold, Craig B. .
OPTICS LETTERS, 2008, 33 (18) :2146-2148
[15]   Two-photon microscopy with simultaneous standard and extended depth of field using a tunable acoustic gradient-index lens [J].
Olivier, Nicolas ;
Mermillod-Blondin, Alexandre ;
Arnold, Craig B. ;
Beaurepaire, Emmanuel .
OPTICS LETTERS, 2009, 34 (11) :1684-1686
[16]   Three-dimensional micro-PTV using deconvolution microscopy [J].
Park, JS ;
Kihm, KD .
EXPERIMENTS IN FLUIDS, 2006, 40 (03) :491-499
[17]   Optically sliced micro-PIV using confocal laser scanning microscopy (CLSM) [J].
Park, JS ;
Choi, CK ;
Kihm, KD .
EXPERIMENTS IN FLUIDS, 2004, 37 (01) :105-119
[18]   Defocusing digital particle image velocimetry and the three-dimensional characterization of two-phase flows [J].
Pereira, F ;
Gharib, M .
MEASUREMENT SCIENCE AND TECHNOLOGY, 2002, 13 (05) :683-694
[19]   Stereoscopic particle image velocimetry [J].
Prasad, AK .
EXPERIMENTS IN FLUIDS, 2000, 29 (02) :103-116
[20]  
Vettenburg T, 2014, NAT METHODS, V11, P541, DOI [10.1038/NMETH.2922, 10.1038/nmeth.2922]