High-speed axial-scanning wide-field microscopy for volumetric particle tracking velocimetry

被引:13
作者
Chen, T. -H. [1 ]
Ault, J. T. [1 ]
Stone, H. A. [1 ]
Arnold, C. B. [1 ]
机构
[1] Princeton Univ, Dept Mech & Aerosp Engn, Princeton, NJ 08544 USA
关键词
ACOUSTIC GRADIENT-INDEX; IMAGE VELOCIMETRY; T-JUNCTION; FLOWS; LENS;
D O I
10.1007/s00348-017-2316-z
中图分类号
TH [机械、仪表工业];
学科分类号
0802 ;
摘要
The ability to understand and visualize complex flow structures in microfluidic and biological systems relies heavily on the resolving power of three-dimensional (3D) particle velocimetry techniques. We propose a simple technique for acquiring volumetric particle information with the potential for microsecond time resolution. By utilizing a fast varifocal lens in a modified wide-field microscope, we capture both volumetric and planar information with microsecond time resolution. The technique is demonstrated by tracking particle motions in the complex, three-dimensional flow in a high Reynolds number laminar flow at a branching arrow-shaped junction.
引用
收藏
页数:7
相关论文
共 27 条
[1]   Vortex-Breakdown-Induced Particle Capture in Branching Junctions [J].
Ault, Jesse T. ;
Fani, Andrea ;
Chen, Kevin K. ;
Shin, Sangwoo ;
Gallaire, Francois ;
Stone, Howard A. .
PHYSICAL REVIEW LETTERS, 2016, 117 (08)
[2]   Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution [J].
Chen, Bi-Chang ;
Legant, Wesley R. ;
Wang, Kai ;
Shao, Lin ;
Milkie, Daniel E. ;
Davidson, Michael W. ;
Janetopoulos, Chris ;
Wu, Xufeng S. ;
Hammer, John A., III ;
Liu, Zhe ;
English, Brian P. ;
Mimori-Kiyosue, Yuko ;
Romero, Daniel P. ;
Ritter, Alex T. ;
Lippincott-Schwartz, Jennifer ;
Fritz-Laylin, Lillian ;
Mullins, R. Dyche ;
Mitchell, Diana M. ;
Bembenek, Joshua N. ;
Reymann, Anne-Cecile ;
Boehme, Ralph ;
Grill, Stephan W. ;
Wang, Jennifer T. ;
Seydoux, Geraldine ;
Tulu, U. Serdar ;
Kiehart, Daniel P. ;
Betzig, Eric .
SCIENCE, 2014, 346 (6208) :439-+
[3]   Vortex dynamics in a pipe T-junction: Recirculation and sensitivity [J].
Chen, Kevin K. ;
Rowley, Clarence W. ;
Stone, Howard A. .
PHYSICS OF FLUIDS, 2015, 27 (03)
[4]   Three-dimensional volumetric measurement of red blood cell motion using digital holographic microscopy [J].
Choi, Yong-Seok ;
Lee, Sang-Joon .
APPLIED OPTICS, 2009, 48 (16) :2983-2990
[5]   Particle imaging techniques for volumetric three-component (3D3C) velocity measurements in microfluidics [J].
Cierpka, C. ;
Kaehler, C. J. .
JOURNAL OF VISUALIZATION, 2012, 15 (01) :1-31
[6]   Image Sharpness Measure for Blurred Images in Frequency Domain [J].
De, Kanjar ;
Masilamani, V. .
INTERNATIONAL CONFERENCE ON DESIGN AND MANUFACTURING (ICONDM2013), 2013, 64 :149-158
[7]   Experimental measurement of large-scale three-dimensional structures in a turbulent boundary layer. Part 2. Long structures [J].
Dennis, David J. C. ;
Nickels, Timothy B. .
JOURNAL OF FLUID MECHANICS, 2011, 673 :218-244
[8]   Simultaneous multiplane confocal microscopy using acoustic tunable lenses [J].
Duocastella, Marti ;
Vicidomini, Giuseppe ;
Diaspro, Alberto .
OPTICS EXPRESS, 2014, 22 (16) :19293-19301
[9]   Simultaneous imaging of multiple focal planes for three-dimensional microscopy using ultra-high-speed adaptive optics [J].
Duocastella, Marti ;
Sun, Bo ;
Arnold, Craig B. .
JOURNAL OF BIOMEDICAL OPTICS, 2012, 17 (05)
[10]   Tomographic particle image velocimetry [J].
Elsinga, G. E. ;
Scarano, F. ;
Wieneke, B. ;
van Oudheusden, B. W. .
EXPERIMENTS IN FLUIDS, 2006, 41 (06) :933-947