A novel electrochemical sensing strategy for rapid and ultrasensitive detection of Salmonella by rolling circle amplification and DNA-AuNPs probe

被引：78

作者：

Zhu, Dan ^{[1
]}

Yan, Yurong ^{[1
]}

Lei, Pinhua ^{[1
]}

Shen, Bo ^{[1
]}

Cheng, Wei ^{[1
,3
]}

Ju, Huangxian ^{[1
,2
]}

Ding, Shijia ^{[1
]}

机构：

[1] Chongqing Med Univ, Coll Lab Med, Minist Educ, Key Lab Clin Lab Diagnost, Chongqing 400016, Peoples R China

[2] Nanjing Univ, Dept Chem, State Key Lab Analyt Chem Life Sci, Nanjing 210093, Jiangsu, Peoples R China

[3] Chongqing Med Univ, Affiliated Hosp 1, Ctr Clin Mol Med Detect, Chongqing 400016, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2014年 / 846卷

基金：

中国国家自然科学基金;

关键词：

Electrochemical biosensor; InvA gene; Salmonella; Rolling circle amplification; Gold nanoparticles; HIGH-SENSITIVE DETECTION; LABEL-FREE; GOLD NANOPARTICLES; TYPHIMURIUM; BACTERIA; APTASENSOR; BIOSENSOR; SEQUENCE; PCR;

D O I：

10.1016/j.aca.2014.07.024

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel electrochemical sensing strategy was developed for ultrasensitive and rapid detection of Salmonella by combining the rolling circle amplification with DNA-AuNPs probe. The target DNA could be specifically captured by probe 1 on the sensing interface. Then the circularization mixture was added to form a typical sandwich structure. In the presence of dNTPs and phi29 DNA polymerase, the RCA was initiated to produce micrometer-long single-strand DNA. Finally, the detection probe (DNA-AuNPs) could recognize RCA product to produce enzymatic electrochemical signal. Under optimal conditions, the calibration curve of synthetic target DNA had good linearity from 10 aM to 10 pM with a detection limit of 6.76 aM (S/N = 3). The developed method had been successfully applied to detect Salmonella as low as 6 CFU mL(-1) in real milk sample. This proposed strategy showed great potential for clinical diagnosis, food safety and environmental monitoring. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：44 / 50

页数：7

共 38 条

[1] Electrochemical detection of Salmonella using gold nanoparticles [J].