Recent advances in high-throughput 13C-fluxomics

被引：50

作者：

Heux, Stephanie ^{[1
]}

Berges, Cecilia ^{[1
]}

Millard, Pierre ^{[1
]}

Portais, Jean-Charles ^{[1
]}

Létisse, Fabien ^{[1
]}

机构：

[1] Univ Toulouse, CNRS, LISBP, INRA,INSA, Toulouse, France

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2017年 / 43卷

关键词：

METABOLIC FLUX ANALYSIS; ISOTOPE LABELING EXPERIMENTS; NUCLEAR-MAGNETIC-RESONANCE; STIRRED-TANK BIOREACTORS; MASS-SPECTROMETRY; CORYNEBACTERIUM-GLUTAMICUM; ESCHERICHIA-COLI; GENOME-SCALE; FRAMEWORK; SOFTWARE;

D O I：

10.1016/j.copbio.2016.10.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The rise of high throughput (HT) strain engineering tools accompanying the area of synthetic biology is supporting the generation of a large number of microbial cell factories. A current bottleneck in process development is our limited capacity to rapidly analyze the metabolic state of the engineered strains, and in particular their intracellular fluxes. HT C-13-fluxomics workflows have not yet become commonplace, despite the existence of several HT tools at each of the required stages. This includes cultivation and sampling systems, analytics for isotopic analysis, and software for data processing and flux calculation. Here, we review recent advances in the field and highlight bottlenecks that must be overcome to allow the emergence of true HT C-13-fluxomics workflows.

引用

页码：104 / 109

页数：6

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