Biochemical characterization of a lichenase from Penicillium occitanis Pol6 and its potential application in the brewing industry

被引：36

作者：

Chaari, Fatma ^{[1
]}

Belghith-Fendri, Lilia ^{[1
]}

Blibech, Monia ^{[1
]}

Driss, Dorra ^{[1
]}

Ellouzi, Soumaya Zaouri ^{[1
]}

Sameh, Maktouf ^{[1
]}

Ellouz-Chaabouni, Semia ^{[1
,2
]}

机构：

[1] Sfax Univ, Sfax Natl Sch Engineers, Dept Biol, Unite Enzymes & Bioconvers, Sfax 3038, Tunisia

[2] Sfax Natl Sch Engineers, Unite Serv Commun Bioreacteur Couple Ultrafiltre, Sfax 3038, Tunisia

来源：

PROCESS BIOCHEMISTRY | 2014年 / 49卷 / 06期

关键词：

Lichenase; Penicillium occitanis; Brewing industry; BETA-GLUCANASE; BETA-1,3-1,4-GLUCANASE LICHENASE; THERMOSTABLE BETA-1,3-1,4-GLUCANASE; 1,3-1,4-BETA-D-GLUCANASE LICHENASE; TALAROMYCES-EMERSONII; BROILER-CHICKENS; PURIFICATION; GENE; OPTIMIZATION; PERFORMANCE;

D O I：

10.1016/j.procbio.2014.02.023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The purification and characterization of an extracellular lichenase from the fungus Penicillium occitanis Pol6 were studied. The strain produced the maximum level of extracellular lichenase (45 +/- 5 U ml(-1)) when grown in a medium containing oat flour (2%, w/v) at 30 degrees C for 7 days. The purified enzyme EG(L) showed as a single protein band on SDS-PAGE with a molecular mass of 20 kDa. Its N-terminal sequence of 10 amino acid residues was determined as LDNGAPLLNV. The purified enzyme showed an optimum activity at pH 3.0 and 50-60 degrees C. The half-lives of EG(L) at 60 degrees C and 70 degrees C were 80 min and 21 min, respectively. Substrate specificity studies revealed that the enzyme is a true beta-1,3-1,4-D-glucanase. The enzyme hydrolyzed lichenan to yield trisaccharide, and tetrasaccharide as the main products. Under simulated mashing conditions, addition of EG(L) (20 U/ml) or a commercial beta-glucanase (20 U/ml) reduced the filtration time (25% and 21.3%, respectively) and viscosity (10% and 8.18%, respectively). These characteristics indicate that EG(L) is a good candidate in the malting and brewing industry. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：1040 / 1046

页数：7

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