Genetic Organization of Transposase Regions Surrounding blaKPC Carbapenemase Genes on Plasmids from Klebsiella Strains Isolated in a New York City Hospital
被引:141
作者:
Gootz, Thomas D.
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Pfizer Global Res & Dev, Dept Infect Dis, Groton, CT 06340 USAPfizer Global Res & Dev, Dept Infect Dis, Groton, CT 06340 USA
Gootz, Thomas D.
[1
]
Lescoe, Mary Kay
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Pfizer Global Res & Dev, Dept Infect Dis, Groton, CT 06340 USAPfizer Global Res & Dev, Dept Infect Dis, Groton, CT 06340 USA
Lescoe, Mary Kay
[1
]
Dib-Hajj, Fadia
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Pfizer Global Res & Dev, Dept Infect Dis, Groton, CT 06340 USAPfizer Global Res & Dev, Dept Infect Dis, Groton, CT 06340 USA
Dib-Hajj, Fadia
[1
]
Dougherty, Brian A.
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Pfizer Global Res & Dev, Mol Biol, Groton, CT 06340 USAPfizer Global Res & Dev, Dept Infect Dis, Groton, CT 06340 USA
Dougherty, Brian A.
[4
]
He, Wen
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Pfizer Global Res & Dev, Mol Biol, Groton, CT 06340 USAPfizer Global Res & Dev, Dept Infect Dis, Groton, CT 06340 USA
He, Wen
[4
]
Della-Latta, Phyllis
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机构:
Columbia Univ, Med Ctr, New York Presbyterian Hosp, Clin Microbiol Serv, New York, NY 10032 USA
Columbia Univ, Med Ctr, New York Presbyterian Hosp, Dept Pathol, New York, NY 10032 USAPfizer Global Res & Dev, Dept Infect Dis, Groton, CT 06340 USA
Della-Latta, Phyllis
[2
,3
]
Huard, Richard C.
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机构:
Columbia Univ, Med Ctr, New York Presbyterian Hosp, Clin Microbiol Serv, New York, NY 10032 USA
Columbia Univ, Med Ctr, New York Presbyterian Hosp, Dept Pathol, New York, NY 10032 USAPfizer Global Res & Dev, Dept Infect Dis, Groton, CT 06340 USA
Huard, Richard C.
[2
,3
]
机构:
[1] Pfizer Global Res & Dev, Dept Infect Dis, Groton, CT 06340 USA
[2] Columbia Univ, Med Ctr, New York Presbyterian Hosp, Clin Microbiol Serv, New York, NY 10032 USA
[3] Columbia Univ, Med Ctr, New York Presbyterian Hosp, Dept Pathol, New York, NY 10032 USA
[4] Pfizer Global Res & Dev, Mol Biol, Groton, CT 06340 USA
Carbapenem-resistant Klebsiella strains carrying Klebsiella pneumoniae carbapenemases (KPC) are endemic to New York City and are spreading across the United States and internationally. Recent studies have indicated that the KPC structural gene is located on a 10-kb plasmid-borne element designated Tn4401. Fourteen Klebsiella pneumoniae strains and one Klebsiella oxytoca strain isolated at a New York City hospital in 2005 carrying either bla(KPC-2) or bla(KPC-3) were examined for isoforms of Tn4401. Ten of the Klebsiella strains contained a 100-bp deletion in Tn4401, corresponding to the Tn4401a isoform. The presence of this deletion adjacent to the upstream promoter region of bla(KPC) in Tn4401a resulted in a different -35 promoter sequence of TGGAGA than that of CTGATT present in isoform Tn4401b. Complete sequencing of one plasmid carrying bla(KPC) from each of three nonclonal isolates indicated the presence of genes encoding other types of antibiotic resistance determinants. The 70.6-kb plasmid from K. pneumoniae strain S9 carrying bla(KPC-2) revealed two identical copies of Tn4401b inserted in an inverse fashion, but in this case, one of the elements disrupted a group II self-splicing intron. In K. pneumoniae strain S15, the Tn4401a element carrying bla(KPC-2) was found on both a large 120-kb plasmid and a smaller 24-kb plasmid. Pulsed-field gel electrophoresis results indicate that the isolates studied represent a heterogeneous group composed of unrelated as well as closely related Klebsiella strains. Our results suggest that endemic KPC-positive Klebsiella strains constitute a generally nonclonal population comprised of various alleles of bla(KPC) on several distinct plasmid genetic backgrounds. This study increases our understanding of the genetic composition of the evolving and expanding role of KPC-producing, healthcare-associated, gram-negative pathogens.
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Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Anderson, K. F.
Lonsway, D. R.
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Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Lonsway, D. R.
Rasheed, J. K.
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Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Rasheed, J. K.
Biddle, J.
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Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Biddle, J.
Jensen, B.
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Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Jensen, B.
McDougal, L. K.
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Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
McDougal, L. K.
Carey, R. B.
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Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Carey, R. B.
Thompson, A.
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Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Thompson, A.
Stocker, S.
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Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Stocker, S.
Limbago, B.
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Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Limbago, B.
Patel, J. B.
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Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
机构:
Univ Paris 11, Fac Med Paris Sud, Hop Bicetre, AP HP,Serv Bacteriol Virol, F-94275 Le Kremlin Bicetre, FranceUniv Paris 11, Fac Med Paris Sud, Hop Bicetre, AP HP,Serv Bacteriol Virol, F-94275 Le Kremlin Bicetre, France
Cuzon, Gaelle
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机构:
Naas, Thierry
Demachy, Marie Claude
论文数: 0引用数: 0
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机构:
Ctr Hosp Meaux, Microbiol Lab, F-77104 Meaux, FranceUniv Paris 11, Fac Med Paris Sud, Hop Bicetre, AP HP,Serv Bacteriol Virol, F-94275 Le Kremlin Bicetre, France
机构:
Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Anderson, K. F.
Lonsway, D. R.
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h-index: 0
机构:
Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Lonsway, D. R.
Rasheed, J. K.
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h-index: 0
机构:
Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Rasheed, J. K.
Biddle, J.
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h-index: 0
机构:
Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Biddle, J.
Jensen, B.
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h-index: 0
机构:
Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Jensen, B.
McDougal, L. K.
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h-index: 0
机构:
Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
McDougal, L. K.
Carey, R. B.
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h-index: 0
机构:
Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Carey, R. B.
Thompson, A.
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h-index: 0
机构:
Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Thompson, A.
Stocker, S.
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h-index: 0
机构:
Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Stocker, S.
Limbago, B.
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h-index: 0
机构:
Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Limbago, B.
Patel, J. B.
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h-index: 0
机构:
Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
机构:
Univ Paris 11, Fac Med Paris Sud, Hop Bicetre, AP HP,Serv Bacteriol Virol, F-94275 Le Kremlin Bicetre, FranceUniv Paris 11, Fac Med Paris Sud, Hop Bicetre, AP HP,Serv Bacteriol Virol, F-94275 Le Kremlin Bicetre, France
Cuzon, Gaelle
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机构:
Naas, Thierry
Demachy, Marie Claude
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Hosp Meaux, Microbiol Lab, F-77104 Meaux, FranceUniv Paris 11, Fac Med Paris Sud, Hop Bicetre, AP HP,Serv Bacteriol Virol, F-94275 Le Kremlin Bicetre, France