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Protein displacement by DExH/D "RNA helicases" without duplex unwinding
被引:204
作者:
Fairman, ME
Maroney, PA
Wang, W
Bowers, HA
Gollnick, P
Nilsen, TW
[1
]
Jankowsky, E
机构:
[1] Case Western Reserve Univ, Dept Biochem, Cleveland, OH 44106 USA
[2] Case Western Reserve Univ, Sch Med, Ctr RNA Mol Biol, Cleveland, OH 44106 USA
[3] SUNY Buffalo, Dept Biol Sci, Buffalo, NY 14260 USA
来源:
关键词:
D O I:
10.1126/science.1095596
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
Members of the DExH/D superfamily of nucleic acid-activated nucleotide triphosphatases are essential for virtually all aspects of RNA metabolism, including pre-messenger RNA splicing, RNA interference, translation, and nucleocytoplasmic trafficking. Physiological substrates for these enzymes are thought to be regions of double-stranded RNA, because several DExH/D proteins catalyze strand separation in vitro. These "RNA helicases" can also disrupt RNA-protein interactions, but it is unclear whether this activity is coupled to duplex unwinding. Here we demonstrate that two unrelated DExH/D proteins catalyze protein displacement independently of duplex unwinding. Therefore, the essential functions of DExH/D proteins are not confined to RNA duplexes but can be exerted on a wide range of ribonucleoprotein substrates.
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页码:730 / 734
页数:5
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