Effects of over-expression of glycerol dehydrogenase and 1,3-propanediol oxidoreductase on bioconversion of glycerol into 1,3-propandediol by Klebsiella pneumoniae under micro-aerobic conditions

被引：41
作者：
Zhao, Li [1 ]
Zheng, Yu [1 ]
Ma, Xingyuan [1 ]
Wei, Dongzhi [1 ]
机构：
[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, New World Inst Biotechnol, Shanghai 200237, Peoples R China
来源：
BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2009年 / 32卷 / 03期
关键词：
1,3-Propanediol; 1,3-Propanediol oxidoreductase; Glycerol dehydrogenase; Klebsiella pneumoniae; Micro-aerobic conditions; FERMENTATION; 3-HYDROXYPROPIONALDEHYDE; DISSIMILATION; OVEREXPRESSION; ACCUMULATION; PURIFICATION; METABOLISM; GENES;
D O I：
10.1007/s00449-008-0250-4
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Glycerol dehydrogenase (GDH) and 1,3-propanediol (1,3-PD) oxidoreductase had been proved two key enzymes for 1,3-PD production by Klebsiella pneumoniae. Fed-batch fermentations of the recombinant K. pneumoniae strains, over-expressing the two enzymes individually, were carried out under micro-aerobic conditions, and the behaviors of the recombinants were investigated. Results showed that over-expression of 1,3-PD oxidoreductase did not affect the concentration of 1,3-PD. However, it enhanced the molar yield from 50.6 to 64.0% and reduced the concentration of by-products. Among them, the concentrations of lactic acid, ethanol and succinic acid were decreased by 51.8, 50.6 and 47.4%, respectively. Moreover, in the recombinant the maximal concentration of 3-hydroxypropionaldehyde decreased by 73.6%. Over-expression of GDH decreased the yield of ethanol and 2,3-butanediol, meanwhile it increased the concentration of acetic acid. No significant changes were observed both in 1,3-PD yield and glycerol flux distributed to oxidative branch.
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页码：313 / 320
页数：8
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