The biological effects of five feline IFN-α subtypes

被引：18

作者：

Baldwin, SL ^{[1
]}

Powell, TD ^{[1
]}

Sellins, KS ^{[1
]}

Radecki, SV ^{[1
]}

Cohen, JJ ^{[1
]}

Milhausen, MJ ^{[1
]}

机构：

[1] Heska Corp, Ft Collins, CO 80525 USA

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2004年 / 99卷 / 3-4期

关键词：

feline IFN-alpha; Mx; feline calicivirus; vesicular stomatitis virus; antiproliferation; necrosis; apoptosis;

D O I：

10.1016/j.vetimm.2004.01.012

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

IFN-alpha has been shown to induce both antiviral and antiproliferative activities in annuals. This report describes the biological activity of five recently identified feline IFN-alpha subtypes expressed in the Chinese hamster ovary (CHO) cell line (rfeIFN-alpha1[CHO], rfeIFN-alpha2[CHO], rfeIFN-alpha3[CHO], rfeIFN-alpha5[CHO] and rfeIFN-alpha6[CHO]) and the feIFN-alpha6 subtype expressed in and purified from Pichia pastoris (rfeIFN-alpha6[P. pastoris]). The rfeIFN-alpha[CHO] subtypes were tested for antiviral activity against either Vesicular stomatitis virus (VSV) or feline calicivirus (FCV) infected feline embryonic fibroblast cell line (AH927) or Crandell feline kidney cell line (CRFK). Antiviral activity was induced against both VSV and FCV infected AH927 cells and VSV infected CRFK cells by all five of the rfeIFN-a[CHO] subtypes and rfeIFN-alpha6[P. pastoris]. In addition, the IFN-alpha inducible Mx gene (associated with antiviral activity) was upregulated in vivo 24 h following treatment with rfeIFN-alpha6[P. pastoris], compared to baseline levels seen prior to treatment. All of the rfeIFN-alpha[CHO] subtypes and rfeIFN-alpha6[P. pastoris] exhibited anti proliferative activity in the FeT-J cell line (an IL-2 independent feline T-cell line). Both necrosis and apoptosis were observed in rfeIFN-alpha6[P. pastoris]-treated FeT-J cells. The rfeIFN-alpha3[CHO] subtype consistently exhibited lower antiviral and antiproliferative activity compared to that observed with the other four rfeIFN-alpha[CHO] subtypes. In summary, this paper demonstrates that five previously described feIFN-alpha subtypes induce both antiviral and antiproliferative activities in vitro and are capable of upregulating the feMx gene in vivo. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：153 / 167

页数：15

共 56 条

[1] The antiviral dynamin family member, MxA, tubulates lipids and localizes to the smooth endoplasmic reticulum [J].