A Novel 6′-N-Aminoglycoside Acetyltransferase, AAC(6′)-Ial, from a Clinical Isolate of Serratia marcescens

被引：5
|
作者：
Tada, Tatsuya [1 ]
Miyoshi-Akiyama, Tohru [2 ]
Shimada, Kayo [1 ]
Dahal, Rajan K. [3 ]
Mishra, Shyam K. [3 ]
Ohara, Hiroshi [4 ]
Kirikae, Teruo [1 ]
Pokhrel, Bharat M. [3 ]
机构：
[1] Natl Ctr Global Hlth & Med, Res Inst, Dept Infect Dis, Tokyo 1628655, Japan
[2] Natl Ctr Global Hlth & Med, Res Inst, Pathogen Microbe Lab, Tokyo 1628655, Japan
[3] Tribhuvan Univ, Inst Med, Dept Microbiol, Kathmandu, Nepal
[4] Natl Ctr Global Hlth & Med, Dept Int Med Cooperat, Tokyo 1628655, Japan
来源：
MICROBIAL DRUG RESISTANCE | 2016年 / 22卷 / 02期
关键词：
RIBOSOMAL-RNA METHYLASE; METALLO-BETA-LACTAMASE; PSEUDOMONAS-AERUGINOSA; IDENTIFICATION; RESISTANCE; AMINOGLYCOSIDES; OUTBREAK; GENES; KOREA;
D O I：
10.1089/mdr.2015.0126
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Serratia marcescens IOMTU115 has a novel 6 '-N-aminoglycoside acetyltransferase-encoding gene, aac(6 ')-Ial. The encoded protein AAC(6 ')-Ial has 146 amino acids, with 91.8% identity to the amino acid sequence of AAC(6 ')-Ic in S. marcescens SM16 and 97.3% identity to the amino acid sequence of AAC(6 ')-Iap in S. marcescens WW4. The minimum inhibitory concentrations of aminoglycosides for Escherichia coli expressing AAC(6 ')-Ial were similar to those for E. coli expressing AAC(6 ')-Ic or AAC(6 ')-Iap. Thin-layer chromatography showed that AAC(6 ')-Ial, AAC(6 ')-Ic, or AAC(6 ')-Iap acetylated all the aminoglycosides tested, except for apramycin, gentamicin, and lividomycin. Kinetics assays revealed that AAC(6 ')-Ial is a functional acetyltransferase against aminoglycosides. The aac(6 ')-Ial gene was located on chromosomal DNA.
引用
收藏
页码：103 / 108
页数：6
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