Euzebyella marina sp nov., isolated from seawater

A Gram-stain-negative, aerobic, yellow-pigmented, non-flagellated, non-gliding, oxidase-and catalase-positive bacterium, designated CY01(T), was isolated from seawater of the Yellow Sea. CY01(T) grew at 15-37 degrees C (optimum, 30 degrees C), pH 5-8 (optimum, 6.5-7.5) and with 0.5-12% (w/v) NaCl (optimum, 0.5-3.5 %). It could not produce flexirubin-type pigment or reduce nitrate to nitrite. CY01(T) showed the highest 16S rRNA gene sequence similarity to the type strain of Euzebyella saccharophila (97.0 %) and clustered tightly with the species of the genus Euzebyella in the phylogenetic trees based on the 16S rRNA gene sequences. The major cellular fatty acids of CY01(T) were iso-C-15:0, iso-C(15:1)G and iso-C-17:0 3-OH and the major respiratory quinone was menaquinone MK-6. Polar lipids included phosphatidylethanolamine (PE), four unidentified lipids and one unidentified aminolipid. The genomic DNA G+C content was 38.2 mol%. Based on the results of the polyphasic characterization of CY01(T), it represents a novel species of the genus Euzebyella, for which the name Euzebyella marina sp. nov. is proposed. The type strain is CY01(T) (=CCTCC AB 2014348(T)=KCTC 42440(T)).