Impact of a wastewater treatment plant on microbial community composition and function in a hyporheic zone of a eutrophic river

被引：71

作者：

Atashgahi, Siavash ^{[1
,2
,3
]}

Aydin, Rozelin ^{[1
]}

Dimitrov, Mauricio R. ^{[1
]}

Sipkema, Detmer ^{[1
]}

Hamonts, Kelly ^{[2
]}

Lahti, Leo ^{[1
,4
]}

Maphosa, Farai ^{[1
]}

Kruse, Thomas ^{[1
]}

Saccenti, Edoardo ^{[5
]}

Springael, Dirk ^{[3
]}

Dejonghe, Winnie ^{[2
]}

Smidt, Hauke ^{[1
]}

机构：

[1] Wageningen Univ, Microbiol Lab, NL-6703 HB Wageningen, Netherlands

[2] Flemish Inst Technol Res VITO, Separat & Convers Technol, B-2400 Mol, Belgium

[3] Katholieke Univ Leuven, Div Soil & Water Management, B-3001 Heverlee, Belgium

[4] Univ Helsinki, Dept Vet Biosci, FI-00014 Helsinki, Finland

[5] Wageningen Univ, Lab Syst & Synthet Biol, NL-6703 HB Wageningen, Netherlands

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

芬兰科学院;

关键词：

CHLORINATED ALIPHATIC-HYDROCARBONS; SEASONAL DYNAMICS; CONTAMINATED GROUNDWATER; BACTERIAL COMMUNITIES; COMMON FEATURES; VINYL-CHLORIDE; SEDIMENT; ATTENUATION; METABOLISM; EFFLUENTS;

D O I：

10.1038/srep17284

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The impact of the installation of a technologically advanced wastewater treatment plant (WWTP) on the benthic microbial community of a vinyl chloride (VC) impacted eutrophic river was examined two years before, and three and four years after installation of the WWTP. Reduced dissolved organic carbon and increased dissolved oxygen concentrations in surface water and reduced total organic carbon and total nitrogen content in the sediment were recorded in the post-WWTP samples. Pyrosequencing of bacterial 16S rRNA gene fragments in sediment cores showed reduced relative abundance of heterotrophs and fermenters such as Chloroflexi and Firmicutes in more oxic and nutrient poor post-WWTP sediments. Similarly, quantitative PCR analysis showed 1-3 orders of magnitude reduction in phylogenetic and functional genes of sulphate reducers, denitrifiers, ammonium oxidizers, methanogens and VC-respiring Dehalococcoides mccartyi. In contrast, members of Proteobacteria adapted to nutrient-poor conditions were enriched in post-WWTP samples. This transition in the trophic state of the hyporheic sediments reduced but did not abolish the VC respiration potential in the post-WWTP sediments as an important hyporheic sediment function. Our results highlight effective nutrient load reduction and parallel microbial ecological state restoration of a human-stressed urban river as a result of installation of a WWTP.

引用

页数：13

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