Minimization of protein adsorption on poly(vinylidene fluoride)

被引：33

作者：

Ademovic, Z ^{[1
]}

Klee, D ^{[1
]}

Kingshott, P ^{[1
]}

Kaufmann, R ^{[1
]}

Höcker, H ^{[1
]}

机构：

[1] Rhein Westfal TH Aachen, Dept Text & Macromol Chem, D-52062 Aachen, Germany

来源：

BIOMOLECULAR ENGINEERING | 2002年 / 19卷 / 2-6期

关键词：

protein adsorption; PEG; PVDF; XPS; surface-MALDI-Tof-MS;

D O I：

10.1016/S1389-0344(02)00020-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Surfaces covered with polyethylene glycol (PEG) have been shown to be biocompatible because PEG yields nonimmunogenicity, nonantigenicity and protein rejection. To produce a biocompatible surface coating, we have developed a method for grafting PEG onto modified poly(vinylidene fluoride) (PVDF) films. The first step was to create carboxy groups on the PVDF surface following covalente coupling of polyethylenimine (PEI) to achieve high density of amino groups. These surface amines were reacted with formyl-terminated PEG's with various molecular weight. The modified PVDF surface was characterized by means of static contact angle measurements, infrared (IR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The influence of the chain length on lysozyme repellence was investigated by means of surface-MALDI-Tof mass spectrometry (Surface-MALDI-Tof-MS). Lysozyme adsorption was significantly suppressed on the PEG 5000 modified PVDF surface. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：177 / 182

页数：6

共 17 条

[1] Grafting poly(ethylene glycol) epoxide to amino-derivatized quartz: Effect of temperature and pH on grafting density
Emoto, K
Harris, JM
VanAlstine, JM
[J]. ANALYTICAL CHEMISTRY, 1996, 68 (21) : 3751 - 3757
[2] Harris J.M., 1992, Polyethylene Glycol: Chemistry and Biological Applications
[3] Irvine DJ, 1998, J BIOMED MATER RES, V40, P498, DOI 10.1002/(SICI)1097-4636(19980605)40:3<498::AID-JBM21>3.0.CO
[4] 2-E
[5] PROTEIN SURFACE INTERACTIONS IN THE PRESENCE OF POLYETHYLENE OXIDE .2. EFFECT OF PROTEIN SIZE
JEON, SI
ANDRADE, JD
[J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1991, 142 (01) : 159 - 166
[6] PROTEIN SURFACE INTERACTIONS IN THE PRESENCE OF POLYETHYLENE OXIDE .1. SIMPLIFIED THEORY
JEON, SI
LEE, JH
ANDRADE, JD
DEGENNES, PG
[J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1991, 142 (01) : 149 - 158
[7] Direct detection of proteins adsorbed on synthetic materials by matrix-assisted laser desorption ionization-mass spectrometry
Kingshott, P
St John, HAW
Griesser, HJ
[J]. ANALYTICAL BIOCHEMISTRY, 1999, 273 (02) : 156 - 162
[8] Kingshott P, 2000, J BIOMED MATER RES, V49, P36, DOI 10.1002/(SICI)1097-4636(200001)49:1<36::AID-JBM5>3.0.CO
[9] 2-B
[10] BLOOD COMPATIBILITY OF POLYETHYLENE OXIDE SURFACES
LEE, JH
LEE, HB
ANDRADE, JD
[J]. PROGRESS IN POLYMER SCIENCE, 1995, 20 (06) : 1043 - 1079

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