Assembling a novel bifunctional cellulase-xylanase from Thermotoga maritima by end-to-end fusion

被引：58

作者：

Hong, Su Young

Lee, Jin Suk

Cho, Kye Man

Math, Renukaradhya K.

Kim, Yong Hee

Hong, Sun Joo

Cho, Yong Un

Kim, Hoon

Yun, Han Dae ^{[1
]}

机构：

[1] Gyeongsang Natl Univ, Div Appl Life Sci, Chinju 660701, South Korea

[2] Gyeongsang Natl Univ, Life Sci Res Inst, Chinju 660701, South Korea

[3] Chinju Natl Univ, Dept Microbiol Engn, Chinju 660758, South Korea

[4] Sunchon Natl Univ, Dept Agr Chem, Sunchon 540742, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 22期

关键词：

bifunctional enzyme; gene fusion; cellulase; xylanase;

D O I：

10.1007/s10529-006-9166-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An artificial, bifunctional, thermostable cellulase-xylanase enzyme from Thermotoga maritima by gene fusion. The fusion protein exhibited both cellulase and xylanase activity when xynA was fused downstream of cel5C but no activities were shown when xynA was fused upstream of cel5C. The enzyme was optimally active at pH 5.0 and 80 degrees C over 30 min. E. coli expressed the fusion enzyme, with an apparent molecular mass of approximately 152 kDa by carboxymethyl cellulose- and xylan-SDS-PAGE.

引用

页码：1857 / 1862

页数：6

共 17 条

[1] Evaluation of a novel bifunctional xylanase-cellulase constructed by gene fusion [J].