Differential regulation of the two metallothionein genes in common carp

被引:7
作者
Hermesz, E [1 ]
Gazdag, AP [1 ]
Ali, KS [1 ]
Nemcsók, J [1 ]
Abrahám, M [1 ]
机构
[1] Univ Szeged, Fac Sci, Dept Biochem, H-6701 Szeged, Hungary
来源
ACTA BIOLOGICA HUNGARICA | 2002年 / 53卷 / 03期
关键词
carp; gene expression; metallothionein mRNAs; metal treatment; RT-PCR;
D O I
10.1556/ABiol.53.2002.3.11
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The expression of two metallothionein (MT) genes was followed in carp (Cyprinus carpio) in vivo during exposure to As and Cu. Changes in the levels of MT-1 and MT-2 mRNA in the liver and kidney were detected by semi-quantitative RT-PCR. The inducibility of the two MT isoforms was tissue- and metal-specific. Regardless of whether As or Cu was applied, the liver was more responsive than the kidney. Copper influenced the expression of both isoforms: the MT-1 and MT-2 mRNA levels increased in both the liver and the kidney in a time- and dose-dependent manner. Arsenic affected mostly the MT-2 expression, while the level of the MT-1 transcript did not change significantly in either organ.
引用
收藏
页码:343 / 350
页数:8
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