Differential regulation of the two metallothionein genes in common carp

被引：7
作者：
Hermesz, E [1 ]
Gazdag, AP [1 ]
Ali, KS [1 ]
Nemcsók, J [1 ]
Abrahám, M [1 ]
机构：
[1] Univ Szeged, Fac Sci, Dept Biochem, H-6701 Szeged, Hungary
来源：
ACTA BIOLOGICA HUNGARICA | 2002年 / 53卷 / 03期
关键词：
carp; gene expression; metallothionein mRNAs; metal treatment; RT-PCR;
D O I：
10.1556/ABiol.53.2002.3.11
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The expression of two metallothionein (MT) genes was followed in carp (Cyprinus carpio) in vivo during exposure to As and Cu. Changes in the levels of MT-1 and MT-2 mRNA in the liver and kidney were detected by semi-quantitative RT-PCR. The inducibility of the two MT isoforms was tissue- and metal-specific. Regardless of whether As or Cu was applied, the liver was more responsive than the kidney. Copper influenced the expression of both isoforms: the MT-1 and MT-2 mRNA levels increased in both the liver and the kidney in a time- and dose-dependent manner. Arsenic affected mostly the MT-2 expression, while the level of the MT-1 transcript did not change significantly in either organ.
引用
收藏
页码：343 / 350
页数：8
相关论文
共 50 条
  • [1] Differential Regulation of the Two Metallothionein Genes in Common Carp
    Edit Hermesz
    Anett P. Gazdag
    K. Said Ali
    J. Nemcsók
    Magdolna Ábrahám
    Acta Biologica Hungarica, 2002, 53 : 343 - 350
  • [2] Expression of Two Metallothionein Genes in Different Brain Regions of Common Carp
    K. Said Ali
    Ágnes Ferencz
    Aranka K. Deér
    J. Nemcsók
    Edit Hermesz
    Acta Biologica Hungarica, 2009, 60 : 149 - 158
  • [3] EXPRESSION OF TWO METALLOTHIONEIN GENES IN DIFFERENT BRAIN REGIONS OF COMMON CARP
    Ali, K. Said
    Ferencz, Agnes
    Deer, Aranka K.
    Nemcsok, J.
    Hermesz, Edit
    ACTA BIOLOGICA HUNGARICA, 2009, 60 (02): : 149 - 158
  • [4] Quantitative PCR analysis of two molluscan metallothionein genes unveils differential expression and regulation
    Dondero, F
    Piacentini, L
    Banni, M
    Rebelo, M
    Burlando, B
    Viarengo, A
    GENE, 2005, 345 (02) : 259 - 270
  • [5] Tissue-specific expression of two metallothionein genes in common carp during cadmium exposure and temperature shock
    Hermesz, E
    Abrahám, M
    Nemcsók, J
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 2001, 128 (03): : 457 - 465
  • [6] Comparative study on the expression of glutathione peroxidase, glutathione reductase, glutathione synthetase and metallothionein genes in common carp during cadmium exposure
    Juhasz, R.
    Ferencz, A.
    Jancso, Z.
    Dugmonits, K.
    Hermesz, E.
    FREE RADICAL BIOLOGY AND MEDICINE, 2012, 53 : S220 - S220
  • [7] DIFFERENTIAL REGULATION OF METALLOTHIONEIN GENES IN RAINBOW-TROUT FIBROBLASTS, RTG-2
    ZAFARULLAH, M
    OLSSON, PE
    GEDAMU, L
    BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1049 (03) : 318 - 323
  • [8] A role for multiple estrogen receptors in immune regulation of common carp
    Szwejser, Ewa
    Maciuszek, Magdalena
    Casanova-Nakayama, Ayako
    Segner, Helmut
    Verburg-van Kemenade, B. M. Lidy
    Chadzinska, Magdalena
    DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2017, 66 : 61 - 72
  • [9] EXPRESSIONS OF HEAT SHOCK AND METALLOTHIONEIN GENES IN THE HEART OF COMMON CARP (CYPRINUS CARPIO): EFFECTS OF TEMPERATURE SHOCK AND HEAVY METAL EXPOSURE
    Ali, K. Said
    Ferencz, Agnes
    Nemcsok, J.
    Hermesz, Edit
    ACTA BIOLOGICA HUNGARICA, 2010, 61 (01): : 10 - 23
  • [10] Expressions of Heat Shock and Metallothionein Genes in the Heart of Common Carp (Cyprinus Carpio): Effects of Temperature Shock and Heavy Metal Exposure
    K. Said Ali
    Ágnes Ferencz
    J. Nemcsók
    Edit Hermesz
    Acta Biologica Hungarica, 2010, 61 : 10 - 23
12345