DNA interactions of a functionalized ruthenium(II) mixed-polypyridyl complex [Ru(bpy)2ppd]2+

被引：258

作者：

Gao, Feng

Chao, Hui ^{[1
]}

Zhou, Feng

Yuan, Yi-Xian

Peng, Bin

Ji, Liang-Nian

机构：

[1] Sun Yat Sen Univ, Key Lab Gene Engn, Minist Educ, State Key Lab Optoelect Mat & Technol,Dept Chem, Guangzhou 510275, Peoples R China

[2] Nanjing Univ, State Key Lab Coordinat Chem, Nanjing 210093, Peoples R China

[3] Tongji Univ, Dept Chem, Shanghai 200092, Peoples R China

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 2006年 / 100卷 / 09期

基金：

高等学校博士学科点专项科研基金; 中国国家自然科学基金;

关键词：

ruthenium(II) complex; polypyridyl ligand; DNA-binding; photocleavage;

D O I：

10.1016/j.jinorgbio.2006.04.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel polypyridyl ligand pteridino[7,6-f][1,10]phenanthroline-1,13(10H,12H)-dione (ppd) and its ruthenium(II) complex [Ru(bpy)(2)ppd](2+) have been synthesized and characterized by elemental analysis, electrospray mass spectra and H-1 NMR. The interaction of the complex with calf thymus DNA was investigated by spectroscopic and viscosity measurements. The results suggest that the complex binds to DNA via an intercalative mode and serves as a molecular "light switch" for DNA. Moreover, the complex has been found to promote the photocleavage of plasmid DNA pBR322 under irradiation at 365 nm. The mechanism studies reveal that singlet oxygen (O-1(2)) and superoxide anion radical (O-2(center dot-)) play a significant role in the photocleavage. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：1487 / 1494

页数：8

共 49 条

[1] Ruthenium(II) complexes of redox-related, modified dipyridophenazine ligands: Synthesis, characterization, and DNA interaction [J].

Ambroise, A ;

Maiya, BG .

INORGANIC CHEMISTRY, 2000, 39 (19) :4256-4263

[2] Photocleavage of nucleic acids [J].

Armitage, B .

CHEMICAL REVIEWS, 1998, 98 (03) :1171-1200

[3] Electro-photo switch and "molecular light switch" devices based on ruthenium(II) complexes of modified dipyridophenazine ligands: Modulation of the photochemical function through ligand design [J].

Arounaguiri, S ;

Maiya, BG .

INORGANIC CHEMISTRY, 1999, 38 (05) :842-843

[4] PHOTOACTIVATED STEREOSPECIFIC CLEAVAGE OF DOUBLE-HELICAL DNA BY COBALT(III) COMPLEXES [J].

BARTON, JK ;

RAPHAEL, AL .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1984, 106 (08) :2466-2468

[5] TRIS(PHENANTHROLINE)RUTHENIUM(II) - STEREOSELECTIVITY IN BINDING TO DNA [J].

BARTON, JK ;

DANISHEFSKY, AT ;

GOLDBERG, JM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1984, 106 (07) :2172-2176

[6] VOLTAMMETRIC STUDIES OF THE INTERACTION OF METAL-CHELATES WITH DNA .2. TRIS-CHELATED COMPLEXES OF COBALT(III) AND IRON(II) WITH 1,10-PHENANTHROLINE AND 2,2'-BIPYRIDINE [J].

CARTER, MT ;

RODRIGUEZ, M ;

BARD, AJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (24) :8901-8911

[7] STUDIES ON INTERACTION OF ANTHRACYCLINE ANTIBIOTICS AND DEOXYRIBONUCLEIC-ACID - EQUILIBRIUM BINDING-STUDIES ON INTERACTION OF DAUNOMYCIN WITH DEOXYRIBONUCLEIC-ACID [J].

CHAIRES, JB ;

DATTAGUPTA, N ;

CROTHERS, DM .

BIOCHEMISTRY, 1982, 21 (17) :3933-3940

[8] DNA interactions with ruthenium(II) polypyridine complexes containing asymmetric ligands [J].

Chao, H ;

Ji, LN .

BIOINORGANIC CHEMISTRY AND APPLICATIONS, 2005, 3 (1-2) :15-28

[9] DNA binding studies of ruthenium(II) complexes containing asymmetric tridentate ligands [J].

Chao, H ;

Mei, WJ ;

Huang, QW ;

Ji, LN .

JOURNAL OF INORGANIC BIOCHEMISTRY, 2002, 92 (3-4) :165-170

[10] NICKEL(III)-PROMOTED DNA CLEAVAGE WITH AMBIENT DIOXYGEN [J].

CHENG, CC ;

ROKITA, SE ;

BURROWS, CJ .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1993, 32 (02) :277-278

← 1 2 3 4 5 →