Improvement of the Process Stability of Arylmalonate Decarboxylase by Immobilization for Biocatalytic Profen Synthesis

被引：13

作者：

Assmann, Miriam ^{[1
]}

Muegge, Carolin ^{[2
]}

Gassmeyer, Sarah Katharina ^{[2
]}

Enoki, Junichi ^{[2
]}

Hilterhaus, Lutz ^{[1
]}

Kourist, Robert ^{[2
]}

Liese, Andreas ^{[1
]}

Kara, Selin ^{[1
]}

机构：

[1] Hamburg Univ Technol, Inst Tech Biocatalysis, Hamburg, Germany

[2] Ruhr Univ Bochum, Jr Res Grp Microbial Biotechnol, Bochum, Germany

来源：

FRONTIERS IN MICROBIOLOGY | 2017年 / 8卷

关键词：

profen; arylmalonate decarboxylase; process stability; immobilization; biocatalysis; enantioselectivity; ENZYME IMMOBILIZATION; PROTEIN; PURIFICATION; EVOLUTION;

D O I：

10.3389/fmicb.2017.00448

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The enzyme arylmalonate decarboxylase (AMDase) enables the selective synthesis of enantiopure (S)-arylpropinates in a simple single-step decarboxylation of dicarboxylic acid precursors. However, the poor enzyme stability with a half-life time of about 1.2 h under process conditions is a serious limitation of the productivity, which results in a need for high catalyst loads. By immobilization on an amino C2 acrylate carrier the operational stability of the (S)-selective AMDase variant G74C/M159L/C188G/V43I/A125P/V156L was increased to a half-life of about 8.6 days, which represents a 158-fold improvement. Further optimization was achieved by simple immobilization of the cell lysate to eliminate the cost- and time intensive enzyme purification step.

引用

页数：8

共 25 条

[1] Production of combi-CLEAs of glycosidases utilized for aroma enhancement in wine [J].

Ahumada, Katherine ;

Urrutia, Paulina ;

Illanes, Andres ;

Wilson, Lorena .

FOOD AND BIOPRODUCTS PROCESSING, 2015, 94 :555-560

[2] Engineering the third wave of biocatalysis [J].

Bornscheuer, U. T. ;

Huisman, G. W. ;

Kazlauskas, R. J. ;

Lutz, S. ;

Moore, J. C. ;

Robins, K. .

NATURE, 2012, 485 (7397) :185-194

[3] Immobilizing enzymes: How to create more suitable biocatalysts [J].

Bornscheuer, UT .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (29) :3336-3337

[4]

Cao L, 2005, CARRIER-BOUND IMMOBILIZED ENZYMES: PRINCIPLES, APPLICATIONS AND DESIGN, P1, DOI 10.1002/3527607668

[5] A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications [J].

Cassimjee, K. Engelmark ;

Kadow, M. ;

Wikmark, Y. ;

Humble, M. Svedendahl ;

Rothstein, M. L. ;

Rothstein, D. M. ;

Backvall, J. -E. .

CHEMICAL COMMUNICATIONS, 2014, 50 (65) :9134-9137

[6] Enzyme immobilization: an overview on techniques and support materials [J].

Datta, Sumitra ;

Christena, L. Rene ;

Rajaram, Yamuna Rani Sriramulu .

3 BIOTECH, 2013, 3 (01) :1-9

[7] Arylmalonate Decarboxylase-Catalyzed Asymmetric Synthesis of Both Enantiomers of Optically Pure Flurbiprofen [J].

Gassmeyer, Sarah Katharina ;

Wetzig, Jasmin ;

Muegge, Carolin ;

Assmann, Miriam ;

Enoki, Junichi ;

Hilterhaus, Lutz ;

Zuhse, Ralf ;

Miyamoto, Kenji ;

Liese, Andreas ;

Kourist, Robert .

CHEMCATCHEM, 2016, 8 (05) :916-921

[8]

Guisan J.M., 2006, IMMOBILIZATION ENZYM

[9] CORRELATION BETWEEN STABILITY OF A PROTEIN AND ITS DIPEPTIDE COMPOSITION - A NOVEL-APPROACH FOR PREDICTING INVIVO STABILITY OF A PROTEIN FROM ITS PRIMARY SEQUENCE [J].

GURUPRASAD, K ;

REDDY, BVB ;

PANDIT, MW .

PROTEIN ENGINEERING, 1990, 4 (02) :155-161

[10] Understanding enzyme immobilisation [J].

Hanefeld, Ulf ;

Gardossi, Lucia ;

Magner, Edmond .

CHEMICAL SOCIETY REVIEWS, 2009, 38 (02) :453-468

← 1 2 3 →