Stable isotope imaging of biological samples with high resolution secondary ion mass spectrometry and complementary techniques

被引：42

作者：

Jiang, H. ^{[1
]}

Favaro, E. ^{[2
]}

Goulbourne, C. N. ^{[3
]}

Rakowska, P. D. ^{[4
,5
]}

Hughes, G. M. ^{[1
]}

Ryadnov, M. G. ^{[4
,6
]}

Fong, L. G. ^{[3
]}

Young, S. G. ^{[3
,7
]}

Ferguson, D. J. P. ^{[8
]}

Harris, A. L. ^{[2
]}

Grovenor, C. R. M. ^{[1
]}

机构：

[1] Univ Oxford, Dept Mat, Oxford OX1 3PH, England

[2] Univ Oxford, Weatherall Inst Mol Med, Oxford OX1 3PH, England

[3] Univ Calif Los Angeles, David Geffen Sch Med, Dept Med, Los Angeles, CA 90095 USA

[4] Natl Phys Lab, Teddington TW11 0LW, Middx, England

[5] UCL, Dept Chem, London, England

[6] Univ Edinburgh, Sch Phys & Astron, Edinburgh, Midlothian, Scotland

[7] Univ Calif Los Angeles, Dept Human Genet, Los Angeles, CA USA

[8] Univ Oxford, John Radcliffe Hosp, Nuffield Dept Clin Lab Sci, Oxford OX1 3PH, England

来源：

METHODS | 2014年 / 68卷 / 02期

基金：

英国工程与自然科学研究理事会;

关键词：

Stable isotope; NanoSIMS; Atomic force microscopy; Backscattered electron imaging; Correlative analysis; SUPPORTED LIPID-BILAYERS; GLUTAMINE-METABOLISM; SUBCELLULAR-DISTRIBUTION; NANOSIMS; MICROSCOPY; PEPTIDE; IDENTIFICATION; LOCALIZATION; PROTEINS; REVEALS;

D O I：

10.1016/j.ymeth.2014.02.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Stable isotopes are ideal labels for studying biological processes because they have little or no effect on the biochemical properties of target molecules. The NanoSIMS is a tool that can image the distribution of stable isotope labels with up to 50 rim spatial resolution and with good quantitation. This combination of features has enabled several groups to undertake significant experiments on biological problems in the last decade. Combining the NanoSIMS with other imaging techniques also enables us to obtain not only chemical information but also the structural information needed to understand biological processes. This article describes the methodologies that we have developed to correlate atomic force microscopy and backscattered electron imaging with NanoSIMS experiments to illustrate the imaging of stable isotopes at molecular, cellular, and tissue scales. Our studies make it possible to address 3 biological problems: (1) the interaction of antimicrobial peptides with membranes; (2) glutamine metabolism in cancer cells; and (3) lipoprotein interactions in different tissues. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：317 / 324

页数：8

共 67 条

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