Optimization of myco-synthesized silver nanoparticles by response surface methodology employing Box-Behnken design

被引:94
作者
Barabadi, Hamed [1 ]
Honary, Soheila [2 ]
Ebrahimi, Pouneh [3 ]
Alizadeh, Ahad [4 ]
Naghibi, Farzaneh [5 ]
Saravanan, Muthupandian [6 ]
机构
[1] Shahid Beheshti Univ Med Sci, Sch Pharm, Dept Pharmaceut Biotechnol, Tehran, Iran
[2] Mazandaran Univ Med Sci, Sch Pharm, Sari, Iran
[3] Golestan Univ, Sch Basic Sci, Dept Chem, Gorgan, Golestan, Iran
[4] ACECR, Royan Inst Reprod Biomed, Dept Epidemiol & Reprod Hlth, Reprod Epidemiol Res Ctr, Tehran, Iran
[5] Shahid Beheshti Univ Med Sci, Sch Tradit Med, Tehran, Iran
[6] Mekelle Univ, Coll Hlth Sci, Inst Biomed Sci, Dept Med Microbiol & Immunol, Mekelle 1871, Ethiopia
关键词
Optimization; silver nanoparticles; Penicillium citrinum; response surface methodology; Box-Behnken design; SCOLICIDAL ACTIVITY; GOLD NANOPARTICLES; GREEN SYNTHESIS; ANTIBACTERIAL; TOOL;
D O I
10.1080/24701556.2019.1583251
中图分类号
O61 [无机化学];
学科分类号
070301 ; 081704 ;
摘要
The objective of current study was to optimize the average diameter size of biosynthesized Silver Nanoparticles (AgNPs) induced by Penicillium citrinum. The AgNPs were prepared with a spherical shape and good monodispersity, determined by scanning electron microscopy (SEM), atomic force microscopy (AFM) and photon correlation spectroscopy (PCS). Besides, response surface methodology (RSM) was conducted to optimize the average diameter size of AgNPs by employing Box-Behnken design (BBD). The effective factors in this process were concentration of AgNO3 (mM) (X-1), pH of solution (X-2), temperature of shaker incubator (degrees C) (X-3), rate of shaker incubator (rpm) (X-4) and time of incubation (hour) (X-5). The R-2 (0.8894) value of the obtained model indicated a good correlation between the observed and predicted values. Overall, it could be concluded that multivariate analysis facilitated to find out the optimum conditions for the biosynthesis of AgNPs induced by P. citrinum with the reduced time and cost.
引用
收藏
页码:33 / 43
页数:11
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